UHPLC-Q-TOF-MS快速检测中成药中4个违法掺入的化学成分

Rapidly Detection of Four Chemical Substances Illegally Added in Traditional Chinese Medicine by UHPLC-Q-TOF-MS

关键词:  UHPLC-Q-TOF-MS  违法掺入  雷尼替丁  苯乙双胍  吡罗昔康  双氯芬酸钠

Key words:  UHPLC-Q-TOF-MS  illegally added  ranitidine  phenformin  piroxicam  diclofenac sodium

摘要:

目的 建立UHPLC-Q-TOF-MS快速检测中成药中违法掺入雷尼替丁、苯乙双胍、吡罗昔康、双氯芬酸钠的方法。方法 采用Agilent ZORBAX SB C₁₈色谱柱（2.1 mm×100 mm，1.8 μm），甲醇-10 mmol·L^-1醋酸铵溶液（含0.1%甲酸）为流动相，梯度洗脱；离子源为ESI模式，正离子检测；利用保留时间，一级、二级质谱碎片信息并结合数据库，对4种违法掺入的化学成分进行定性与定量测定。结果 百草糖胶囊中检出雷尼替丁、苯乙双胍；孟氏咳喘灵胶囊中检出雷尼替丁、吡罗昔康、双氯芬酸钠成分。结论 该方法准确可靠，可高效、快速检测中成药中违法掺入的雷尼替丁、苯乙双胍、吡罗昔康、双氯芬酸钠。

Abstract:

OBJECTIVE To establish a method for rapidly detection of ranitidine, phenformin, piroxicam and diclofenac sodium illegally added in Chinese patent medicine by UHPLC-Q-TOF-MS. METHODS The Agilent ZORBAX SB C₁₈(2.1 mm×100 mm, 1.8 μm) column was used, with the mobile phase of methanol-10 mmol·L^-1 ammonium acetate (containing 0.1% formic acid) by gradient elution. Electrospray ionization(ESI) source was used in a positive ion mode, the qualitative and quantitative analyses of the four illegally added substances were combined with retention time, mass spectra, tandem mass spectra and database. RESULTS Ranitidine and phenformin were detected illegally added in Baicaotang capsules. Ranitidine, piroxicam and diclofenac sodium were detected illegally added in Mengshi Kechuanling capsules. CONCLUSION This method is accurate, efficient, rapid and can be used to detect the ranitidine, phenformin, piroxicam and diclofenac sodium illegally added in Chinese patent medicine.