UPLC-MS/MS测定安神类中成药和保健食品中8种非法添加抗抑郁类化学成分

Determination of Eight Antidepressants Illegally Added In Tranquilizing Traditional Chinese Medicine and Health Food by UPLC-MS/MS

关键词:  中成药  保健食品  质谱  超高效液相色谱  抗抑郁

Key words:  traditional Chinese medicine  health food  MS  UPLC  antidepressive

摘要:

目的 建立了安神类中成药和保健食品中的8种非法添加抗抑郁类化学成分的超高效液相色谱-三重四级杆串联质谱（UPLC-MS/MS）检测方法。方法 以甲醇为提取溶剂，Waters ACQUITY UPLC BEH C₁₈（2.1 mm×100 mm，1.7 μm）色谱柱分离，三重四级杆质谱仪检测，MRM模式定性、定量检测安神类中成药和保健食品中的8种抗抑郁类化学成分（文拉法辛、西酞普兰、帕罗西汀、阿米替林、氟西汀、三甲丙咪嗪、舍曲林和氯丙咪嗪）。结果 8种抗抑郁类化学成分质谱检测的线性范围宽，相关性好，r ≥ 0.995 5；方法精密度RSD为1.5%~4.6%；方法回收率为88.2%~103.2%；定量限为0.02~0.03 μg·L^-1；日内精密度的RSD （n=5）为1.1%~3.8%，日间精密度的RSD （n=9）为1.4%~3.7%。结论 本方法专属性强，操作简单，快捷，可作为安神类中成药和保健食品中8种抗抑郁类化学成分定量、定性的有效检测方法。

Abstract:

OBJECTIVE To develop a ultra performance liquid chromatography coupled with triple quadrupole mass spectrometry(UPLC-MS/MS) method to determine 8 antidepressants in tranquilizing traditional Chinese medicine and health food. METHODS The analysis was performed by a UPLC-MS/MS system with Waters ACQUITY UPLC BEH C₁₈ (2.1 mm×100 mm, 1.7 μm) column. Multiple-reaction monitoring (MRM) was performed to identify and quantify 8 antidepressants(venlafaxine, citalopram, paroxetine, amitriptyline, trimepramine, fluoxetine, sertraline and clomipramine), which were extracted from samples with methanol. RESULTS Linear calibration curves were obtained with r ≥ 0.995 5. The precision of the method was showed by RSDs (n=6) ranging from 1.5% to 4.6%. The recoveries were determined at three concentration levels and ranged from 88.2% to 103.2%. The ranges of LOQs were from 0.02 to 0.03 μg·L^-1 and the RSDs(n=9) of intra-day precision and inter-day precision were from 1.1% to 3.8% and from 1.4% to 3.7%, respectively. CONCLUSION The method is specific, simple and rapid to detect 8 antidepressants in tranquilizing traditional Chinese medicine and health food.