不同产地白及遗传多态性的RAPD分析

RAPD Analysis for Genetic Diversity of Bletilla Striata from Different Habitats

关键词:  白及  随机扩增多态DNA  聚类分析  遗传多样性

Key words:  Bletilla striata  RAPD  cluster analysis  genetic diversity

摘要:

目的 研究国内不同产地白及的遗传多样性和亲缘关系。方法 利用随机扩增多态DNA （random amplified polymorphic DNA，RAPD）引物，RAPD分子标记技术分析国内50个不同产地的白及。结果 RAPD技术扩增产物经琼脂凝胶电泳检测，从50条引物中筛选出10条（S8，S9，S14，S19，S23，S25，S28，S29，S30，S31）条带清晰的引物，10条引物共扩增出88条DNA条带，其中多态性的DNA条带数目为81条，占总数的92.05%。每个引物能扩增出5~11条DNA条带，平均可扩增出8.8条；扩增最少的引物为S19，扩增出5条DNA条带；扩增最多的引物为S29，扩增出11条DNA条带。而每个引物能扩增的多态性DNA条带数为3~11条，平均可扩增出8.6条。结论 不同产地的白及具有较丰富的遗传多样性，RAPD可有效应用于白及的遗传多样性研究。

Abstract:

Objective To explore the genetic diversities and relationship of Bletilla striata from different habitats. Methods random amplified polymorphic DNA(RAPD) primers were used to analyze the Bletilla striata from 50 different areas with RAPD molecular marker technique. Results The clear bands of S8, S9, S14, S19, S23, S25, S28, S29, S30 and S31 amplified by RAPD molecular marker technique and analyzed by agarose gel electrophoresis were screened from 50 primers. The 88 DNA fragments were amplified by these 10 primers and 92.05% of these DNA fragments were polymorphic. Each primer was able to amplify 5 to 11 DNA fragments with an average of 8.8 amplified. The amplification number of S19 primers was the least, only 5 fragments and the amplification number of S29 primers was the most with 11 fragments. Each primer was able to amplify 3 to 11 polymorphic DNA fragments with an average of 8.6 amplified. Conclusion There is obvious polymorphism and genetic difference among the Bletilla striata from different areas. RAPD markers exhibite efficiencies for fingerprinting Bletilla sfriata genotypes.