HPLC测定早产儿咖啡因血药浓度

Determination of Caffeine Plasma Concentration of Pemature Infant by HPLC

关键词:  咖啡因  血药浓度监测  早产儿  高效液相色谱法

Key words:  caffeine  plasma concentration monitor  premature infant  HPLC

摘要:

目的 建立HPLC测定早产儿咖啡因血药浓度的方法。方法 色谱柱为Insertil ODS-3（250 mm×4.6 mm，5 μm），甲醇-水（28：72）为流动相，流速1 mL·min^-1，检测波长274 nm，柱温30℃，进样量20 μL。结果 咖啡因血药浓度在2.5~60 mg·L^-1内呈良好的线性关系（r²=0.999 9，n=9），定量限为0.125 mg·L^-1。提取回收率为82.5%~89.7%，方法回收率为97.2%~103.0%；日内与日间精密度RSD均<10%。随机检测5名负荷剂量给药的患儿次日咖啡因谷浓度为（15.8±2.1） mg·L^-1，另5例患儿给药7 d后检测咖啡因谷浓度为（24.3±4.6） mg·L^-1，均在有效线性范围内。结论 本方法灵敏、可靠，可作为咖啡因血药浓度的常规监测方法。

Abstract:

OBJECTIVE To establish HPLC determination method for the caffeine plasma concentration of premature infant. METHODS Chromatographic column was Insertil ODS-3(250 mm×4.6 mm, 5 μm) column with mobile phase consisted of methanol-water(28:72). Flow rate was set at 1 mL·min^-1 with column temperature of 30℃ and detection wavelength was 274 nm. Injection volume was 20 μL. RESULTS The calibration range of caffeine was 2.5-60 mg·L^-1 (r²=0.999 9, n=9). Detection limit was 0.125 mg·L^-1. The average recoveries of extraction and method were 82.5%-89.7% and 97.2%-103.0%. RSDs of intra-day and inter-day were<10%. Caffeine valley concentration of five infants randomly selected with loading-dose were (15.8±2.1)mg·L^-1, while in other five infant were (24.3±4.6)mg·L^-1 after caffeine administration for 7 d. All the results were within methodological linear limitation. CONCLUSION The method is rapid, simple and accurate to be used for plasma concentration monitoring of caffeine.