HPLC同时测定坤泰胶囊中毛蕊花糖苷、芍药苷和黄芩苷含量

Simultaneous Determination of Verbascoside, Paeoniflorin and Baicalin in Kuntai Capsules by HPLC

关键词:  坤泰胶囊  毛蕊花糖苷  芍药苷  黄芩苷  含量测定

Key words:  Kuntai capsules  verbascoside  paeoniflorin  baicalin  content determination

摘要:

目的 建立HPLC同时测定坤泰胶囊中毛蕊花糖苷、芍药苷和黄芩苷含量的方法，为完善现有质量标准提供参考。方法 采用Phenomsil C₁₈（4.6 mm×250 mm，5 μm）色谱柱；以乙腈为流动相A，以0.1%乙酸为流动相B，采用梯度洗脱；流速为1.0 mL·min^-1；柱温为35℃；毛蕊花糖苷的检测波长为334 nm，芍药苷检测波长为230 nm，黄芩苷的检测波长为280 nm。结果 毛蕊花糖苷在3.27~32.68 μg·mL^-1内线性关系良好，平均回收率为98.4%，RSD为0.68%（n=6）；芍药苷在17.65~176.51μg·mL^-1内线性关系良好，平均回收率为99.2%，RSD为1.08%（n=6）；黄芩苷在48.78~487.77 μg·mL^-1内线性关系良好，平均回收率为97.7%，RSD为0.87%（n=6）。结论 该方法简便、快捷、结果准确、重复性好，可应用于坤泰胶囊的质量控制。

Abstract:

OBJECTIVE To establish an HPLC method for simultaneous determination of verbascoside, paeoniflorin and baicalin in Kuntai capsules, provide reference for the improvement of the existing quality standards. METHODS Using Phenomsil C₁₈(4.6 mm×250 mm, 5 μm) chromatography column; mobile phase was acetonitrile(A)-0.1% acetic(B) with gradient elution; flow rate was 1.0 mL·min^-1; column temperature was 35℃; the detection wavelength of verbascoside, paeoniflorin and baicalin was 334, 230, 280 nm, respectively. RESULTS Verbascoside had good linear relationship in 3.27-32.68 μg·mL^-1, the average recovery was 98.4%, RSD was 0.68%(n=6). Paeoniflorin had good linear relationship in 17.65-176.51 μg·mL^-1, the average recovery was 99.2%, RSD was 1.08%(n=6). Baicalin had good linear relationship in 48.78-487.77 μg·mL^-1, the average recovery was 97.7%, RSD was 0.87%(n=6). CONCLUSION The method is simple, shortcut, accurate, repeatable and can be used for the quality control of Kuntai capsules.