番薯藤提取物抗痤疮丙酸杆菌有效部位筛选研究

Study on Anti-propionibacterium Acne Effective Parts of Sweet Potato Vines Extracts

关键词:  番薯藤  痤疮丙酸杆菌  系统溶剂法  固体平板法  试管二倍稀释法

Key words:  sweet potato vines  Propionibacterium acne  systematic solvents method  agar dilution method  liquid media two fold dilution method

摘要:

目的 研究番薯藤提取物对痤疮丙酸杆菌的体外抑菌作用，并对其有效部位进行筛选。方法 采用固体平板法、试管2倍比稀释法测定番薯藤水、醇提物对痤疮丙酸杆菌的抑菌圈大小和最低抑菌浓度（minimum inhibitory concentration，MIC），采用系统溶剂法对番薯藤醇提物进行梯度提取，分别得到石油醚层、二氯甲烷层、乙酸乙酯层、正丁醇层和水层5个不同极性部位，并确定其有效部位及相应的MIC、最低杀菌浓度（minimum bactericidal concentration，MBC）。结果 番薯藤醇提物对痤疮丙酸杆菌有较强抑制作用，其MIC为50 mg·mL^-1。其中石油醚部位的抑菌作用最强，MIC为12.5 mg·mL^-1，MBC为25 mg·mL^-1；其次为正丁醇部位，MIC为25 mg·mL^-1，MBC为100 mg·mL^-1。结论 番薯藤醇提物对痤疮丙酸杆菌存在体外抑制作用，其有效成分主要集中于石油醚和正丁醇部位。

Abstract:

OBJECTIVE To study the bacteriostasis of sweet potato vines extract to Propionibacterium acne and screen the active fractions. METHODS Using agar dilution method and liquid media two fold dilution method to determine the size of the inhibitory rings and the minimal inhibitory concentration(MIC) of the sweet potato vines water extract and alcohol extract to P. acnes. Using the system solvent method to gradient extract sequence of sweet potato vine alcohol extract, respectively got petroleum ether layer, dichloromethane layer, ethyl acetate layer, N-butyl alcohol layer and water layer such five different polar parts and to determine the active fractions and relevant MIC and minimum bactericidal concentration(MBC). RESULTS The sweet potato vines alcohol extract showed strong bacteriostasis to P. acnes, and the MIC was 50 mg·mL^-1. The petroleum ether part showed the strongest bacteriostasis with the MIC of 12.5 mg·mL^-1 and the MBC of 25 mg·mL^-1, followed by N-butyl alcohol part with the MIC of 25 mg·mL^-1 and the MBC of 100 mg·mL^-1. CONCLUSION The sweet potato vines alcohol extract has strong bacteriostasis to P. acnes in vitro, and the antibacterial active ingredients mainly concentrate in petroleum ether and N-butyl alcohol parts.