HPLC-DAD同时测定益心通胶囊中6种活性成分的含量

Simultaneous Determination of Six Kinds of Components in Yixintong Capsules by HPLC-DAD

关键词:  高效液相色谱-二极管阵列检测器  益心通胶囊  葛根素  龙胆苦苷  阿魏酸  毛蕊花糖苷  丹酚酸B  丹皮酚  含量

Key words:  HPLC-DAD  Yixintong capsules  puerarin  gentiopicroside  ferulic acid  acteoside  salvianolic acid B  paeonol  content

摘要:

目的 建立HPLC同时测定益心通胶囊中葛根素、龙胆苦苷、阿魏酸、毛蕊花糖苷、丹酚酸B和丹皮酚6种活性成分含量的方法。方法 采用XSELECT^TM HSS C₁₈色谱柱，流动相为乙腈（A）-0.2%磷酸水溶液（B），梯度洗脱；柱温：30℃；流速：1.0 mL·min^-1；检测波长：阿魏酸、毛蕊花糖苷和丹酚酸B为220 nm，葛根素为250 nm，龙胆苦苷和丹皮酚为275 nm。结果 葛根素、龙胆苦苷、阿魏酸、毛蕊花糖苷、丹酚酸B和丹皮酚的线性范围分别为7.56~113.4 μg·mL^-1（r=0.999 8），4.36~65.4μg·mL^-1（r=0.999 7），1.18~17.7 μg·mL^-1（r=0.999 3），1.90~28.5 μg·mL^-1（r=0.999 4），4.48~67.2 μg·mL^-1（r=0.999 3）和11.7~175.5 μg·mL^-1（r=0.999 7），平均加样回收率分别为98.7%，97.8%，97.6%，98.6%，99.0%，98.7%，RSD分别为1.0%，1.3%，1.4%，1.1%，1.2%，1.3%。结论 本法专属性强，结果准确，重现性好，可用于益心通胶囊的质量控制。

Abstract:

Objective To establish an HPLC-DAD method for determination of six bioactive components in Yixintong capsules, including puerarin, gentiopicroside, ferulic acid, acteoside, salvianolic acid B and paeonol. Methods A stable HPLC method was established and the chromatography was accomplished on a XSELECT^TM HSS C₁₈ column, the mobile phase consisted of methanol(A)-0.2% phosphoric acid(B) in a gradient mode. The flow rate was 1.0 mL·min^-1 and the column temperature was set at 30℃. The detection wavelength was set at 220 nm for paeoniflorin, acteoside and salvianolic acid B, 250 nm for puerarin, 275 nm for gentiopicroside and paeonol, respectively. Results The linear ranges of puerarin, gentiopicroside, paeoniflorin, acteoside, salvianolic acid B and paeonol were 7.56-113.4 μg·mL^-1(r=0.999 8), 4.36-65.4 μg·mL^-1(r=0.999 7), 1.18-17.7 μg·mL^-1(r=0.999 3), 1.90-28.5 μg·mL^-1(r=0.999 4), 4.48-67.2 μg·mL^-1(r=0.999 3) and 11.7-175.5 μg·mL^-1(r=0.999 7), respectively. The average recoveries were 98.7%, 97.8%, 97.6%, 98.6%, 99.0% and 98.7%, RSD were 1.0%, 1.3%, 1.4%, 1.1%, 1.2% and 1.3%. Conclusion The method is specificity, accurate and reproducible, which can be used for the quality control of Yixintong capsules.