| 引用本文: | 聂昌平,吴丹,杨俊,冉海霞,杨青,陈晶,梁光平.制备型高效液相色谱法制备巴利森苷B对照品[J].中国现代应用药学,2019,36(9):1055-1058. |
| NIE Changping,WU Dan,YANG Jun,RAN Haixia,YANG Qing,CHEN Jing,LIANG Guangping.Preparation of Parishin B Reference Substance by Preparative HPLC[J].Chin J Mod Appl Pharm(中国现代应用药学),2019,36(9):1055-1058. |
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| 摘要: |
| 目的 制备用于含量测定的巴利森苷B对照品。方法 采用大孔吸附树脂柱和硅胶柱色谱结合制备型高效液相色谱分离获得巴利森苷B，并通过紫外、红外、质谱、核磁共振波谱数据鉴定其结构，薄层色谱法和高效液相色谱法检测其纯度，最后在温度、光照等变化下考察其稳定性。结果 从天麻中分离制备了符合含量测定用要求的巴利森苷B对照品，HPLC检测质量分数>99.0%。结论 本研究制备的巴利森苷B可作为对照品，用于含有该成分的天然产物及其制剂的质量评价和药效物质基础研究。 |
| 关键词: 巴利森苷B 天麻 制备型高效液相色谱法 |
| DOI：10.13748/j.cnki.issn1007-7693.2019.09.005 |
| 分类号:R284.2 |
| 基金项目:贵州省科技计划项目（黔科合LH字[2016]7418号、[2017]7302号、[2017]7310号）；遵义医药高等专科学校博士科研启动经费项目（遵医专科合BS2018001号）；遵义医药高等专科学校项目（遵医专科合[2017]007号、[2017]010号） |
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| Preparation of Parishin B Reference Substance by Preparative HPLC |
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NIE Changping1, WU Dan2, YANG Jun1, RAN Haixia1, YANG Qing1, CHEN Jing1, LIANG Guangping1
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1.Department of Pharmacy, Zunyi Medical and Pharmaceutical College, Zunyi 563006, China;2.Zunyi Fifth People's Hospital, Zunyi 563000, China
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| Abstract: |
| OBJECTIVE To prepare parishin B reference substance for quantitative analysis. METHODS Parishin B was isolated and purified by macroporous resin column chromatography, silica gel column chromatography, and preparative HPLC. Its structure was identified by UV, IR, ESI-MS and NMR spectral techniques. The purity was determined by HPLC and TLC. Finally, the stability was investigated under different temperature and illumination. RESULTS The reference substance of parishin B was prepared for Gastrodia elata and its purity was >99.0% in line with the standard of quantitative analysis. CONCLUSION Parishin B prepared by this method can be used as reference substance to the quality control for natural product and their preparations, as well as the research of pharmacodynamic material basis. |
| Key words: parishin B Gastrodia elata preparative HPLC |
