小檗胺对肝癌SMMC-7721细胞侵袭转移的影响

Effect of Berbamine on Migration and Invasion of Human Liver Cancer SMMC-7721 Cells

关键词:  肝癌  小檗胺  SMMC-7721细胞  侵袭  转移  上皮间质转化

Key words:  hepatocellular carcinoma  berbamine  SMMC-7721 cell  migration  invasion  epithelial mesenchymal transition

摘要:

目的 研究小檗胺对人肝癌SMMC-7721细胞侵袭转移作用的影响及其可能的机制。方法 在体外用不同浓度小檗胺处理肝癌SMMC-7721细胞，采用MTT法检测细胞活力的变化；采用细胞划痕试验、Transwell迁移和侵袭试验检测肿瘤细胞侵袭转移能力；采用Western blot法检测细胞上皮间质转化相关蛋白E-cadherin和Vimentin的表达。结果 MTT结果显示浓度<20 μmol·L^-1的小檗胺对SMMC-7721细胞存活率无显著影响。划痕试验和Transwell结果显示，小檗胺呈浓度依赖性抑制肝癌SMMC-7721细胞迁移和侵袭过程。Western blot结果表明，小檗胺显著增强SMMC-7721细胞中E-cadherin表达，而减弱Vimentin表达。结论 人肝癌SMMC-7721细胞侵袭转移能力可被小檗胺抑制，其机制可能与其调控上皮间质转化相关蛋白的表达有关。

Abstract:

OBJECTIVE To study the effect and possible mechanism of berbamine on migration and invasion of human liver cancer SMMC-7721 cells. METHODS After SMMC-7721 cells were incubated with different concentration berbamine in vitro. The cell survival rate was assessed by MTT assay. The effect of berbamine on the migration and invasion of SMMC-7721 cells were detected by wound healing and transwell assays. The expression of E-cadherin and Vimentin protein involved in epithelial mesenchymal transition (EMT) was determined by Western blot assay. RESULTS MTT results showed berbamine exhibited no effect on SMMC-7721 cell survival rate in a range of concentrations. Both wound healing and transwell results showed that berbamine inhibited the migration and invasion of SMMC-7721 cells in a concentration-dependent manner. The Western blot assay confirmed that berbamine could decrease the expression of Vimentin, and increase the expression of E-cadherin. CONCLUSION Berbamine could influence the SMMC-7721 cells migration and invasion, which might be associated with the regulation of the expression of proteins involved in EMT.