| 引用本文: | 业康,韩月丹,毛培江,王志安,黄晶晶,栾会玲,金捷.制草乌配方颗粒与饮片煎剂对H9c2心肌细胞毒性的比较研究[J].中国现代应用药学,2019,36(13):1622-1626. |
| YE Kang,HAN Yuedan,MAO Peijiang,WANG Zhian,HUANG Jingjing,LUAN Huiling,JIN Jie.Comparative Study on Toxicity of Aconiti Kusnezoffii Radix Cocta Formula Granule and Decoctum on H9c2 Myocardial Cell[J].Chin J Mod Appl Pharm(中国现代应用药学),2019,36(13):1622-1626. |
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| 制草乌配方颗粒与饮片煎剂对H9c2心肌细胞毒性的比较研究 |
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业康1, 韩月丹2,3, 毛培江1, 王志安1, 黄晶晶1, 栾会玲1, 金捷1
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1.浙江省中药研究所有限公司, 杭州 310023;2.浙江中医药大学第四临床医学院, 杭州 310053;3.杭州市第一人民医院, 杭州 310006
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| 摘要: |
| 目的 比较相同生药量浓度下制草乌中药配方颗粒与饮片煎剂对H9c2心肌细胞毒性的差异。方法 以不同浓度制草乌配方颗粒与同批次原料饮片煎剂作用于H9c2心肌细胞，MTT法检测细胞活性；Hoechest 33258荧光染色法检测细胞核形态并半定量统计分析；乳酸脱氢酶（lactate dehydrogenase，LDH）试剂盒测定细胞LDH释放率。结果 H9c2细胞在制草乌饮片煎剂的干预下，高剂量组细胞活力下降，LDH释放率提高，且呈浓度依赖，较配方颗粒组有显著性差异；Hoechst 33258荧光染色下可见细胞核固缩碎裂等，相同浓度下饮片煎剂组荧光强度强于配方颗粒组（P<0.05）。结论 在相同生药浓度下，制草乌中药配方颗粒对H9c2心肌细胞的毒性显著小于制草乌饮片煎剂。 |
| 关键词: 制草乌 配方颗粒 饮片煎剂 毒性 H9c2心肌细胞 |
| DOI：10.13748/j.cnki.issn1007-7693.2019.13.005 |
| 分类号:R285.5 |
| 基金项目:浙江省科技计划项目（2018F10079） |
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| Comparative Study on Toxicity of Aconiti Kusnezoffii Radix Cocta Formula Granule and Decoctum on H9c2 Myocardial Cell |
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YE Kang1, HAN Yuedan2,3, MAO Peijiang1, WANG Zhian1, HUANG Jingjing1, LUAN Huiling1, JIN Jie1
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1.Zhejiang Research Institute of Chinese Medicine Co., Ltd., Hangzhou 310023, China;2.The Fourth Clinical Medical College of Zhejiang University of Traditional Chinese Medicine, Hangzhou 310053, China;3.Hangzhou First People's Hospital, Hangzhou 310006, China
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| Abstract: |
| OBJECTIVE To compare the toxicity of Aconiti Kusnezoffii Radix Cocta formula granule and decoctum on H9c2 myocardial cells under the same dosage. METHODS Different concentrations of Aconiti Kusnezoffii Radix Cocta formula granule and decoctum were applied to H9c2 myocardial cells, MTT assay was used to detect cell activity; Hoechst 33258 staining was used to detect cell nucleus morphology and semi quantitative analyze its fluorescence intensity. The release rate of lactate dehydrogenase(LDH) was analyzed by using LDH kits. RESULTS The activity of H9c2 myocardial cells decreased under the action of Aconiti Kusnezoffii Radix Cocta decoctum, the release rate of LDH increased and appeared to be concentration dependent, there was a significant difference in high concentration group with formula granule group. The cell nucleus were shrunken and fragmented under Hoechst 33258 staining, fluorescence intensity significant increased in decoctum group compared with the formula granule group under the same crude drug concentration(P<0.05). CONCLUSION The toxicity of Aconiti Kusnezoffii Radix Cocta formula granule to H9c2 myocardial cells is significantly less than its decoctum under the same crude drug concentrations. |
| Key words: Aconiti Kusnezoffii Radix Cocta formula granule decoctum toxicity H9c2 myocardial cells |
