复方龙胆合剂对小鼠激光损伤后皮肤屏障功能修复的研究

Effect of Fufang Longdan Compound Preparation on Skin Barrier Function After Laser Injury in Mice

关键词:  复方龙胆合剂  皮肤损伤  屏障  炎症

Key words:  Fufang Longdan compound preparation  skin injury  barrier  inflammation

摘要:

目的 研究复方龙胆合剂对于小鼠皮肤激光损伤后屏障修复的作用。方法 选择40只BALB/c小鼠，将小鼠背部脱毛后随机选取10只为正常组，其他30只使用Q开关1064激光机，以5 Hz频率、3 mm光斑、6 J能量照射脱毛区，构建激光损伤皮肤模型。将激光损伤模型小鼠随机分为模型组、对照组、实验组。模型组小鼠不做处理，对照组小鼠单一使用基质涂抹，实验组小鼠在基质涂抹的基础上每日灌胃给药复方龙胆合剂。分别在干预后6，24，48 h，7，14 d测试小鼠皮肤含水量、经皮失水量、小鼠皮肤中增殖细胞核抗原染色，ELISA法检测皮肤组织中髓样分化因子88（myeloid differentiation factor 88，MyD88）、白细胞介素6（interleukin-6，IL-6）的蛋白表达，RT-QPCR检测皮肤中表皮抗菌肽S100a8、S100a9、MyD88以及IL-6的mRNA水平。结果 模型组和对照组在皮肤含水量和经皮失水量的比较中无显著统计学意义，而实验组皮肤含水量显著高于模型组、经皮失水量显著低于模型组（P<0.05）。实验组增殖细胞核抗原表达较强。模型组和对照组皮肤中MyD88、IL-6的蛋白水平以及S100a8、S100a9、MyD88以及IL-6的mRNA水平无统计学意义，但是显著高于正常组（P<0.05），而实验组皮肤中MyD88、IL-6的蛋白水平，S100a8、S100a9、MyD88以及IL-6的mRNA水平显著低于模型组（P<0.05）。结论 复方龙胆合剂可以促进小鼠皮肤激光损伤后屏障功能的恢复，其作用和炎症抑制相关。

Abstract:

OBJECTIVE To study the effect of Fufang Longdan compound preparation on barrier repair of mice after laser injury. METHODS Forty BALB/c mice were selected, ten mice were set as normal group and the other 30 mice's back was depilated by Q switch 1064 laser. The laser damaged skin model was constructed with 5 Hz frequency, 3 mm spot and 6 J energy. Laser damaged spin model were randomly divided into model group, control group and experimental group. Mice in the model group were not treated. The control group was coated with a single substrate. The mice in the experimental group were given Fufang Longdan compound preparation daily on the basis of matrix smear. The skin moisture, percutaneous fluid loss and proliferating cell nuclear antigen staining in mice skin were tested at 6, 24, 48 h, 7, 14 d after intervention. ELISA was used to detect the protein expression of myeloid differentiation factor 88(MyD88) and interleukin 6(IL-6) in skin tissue. RT-QPCR was used to detect the mRNA levels of epidermal antibacterial peptide S100a8, S100a9, MyD88 and IL-6 in skin. RESULTS There was no significant statistical significance between the model group and the control group in the comparison of skin water content and water loss in the skin, but the skin moisture in the experimental group was significantly higher than that in the model group and the water loss in the skin was significantly lower than that in the model group(P<0.05). The expression of proliferating cell nuclear antigen was stronger in the experimental group. The levels of MyD88 and IL-6 and mRNA level of S100a8, S100a9 in the skin of the model group and control group were not statistically significant, but were significantly higher than those of the normal group(P<0.05). The protein level of MyD88 and IL-6 in the skin of the experimental group, the mRNA level of S100a8, S100a9, MyD88 and IL-6 in the skin of the experimental group was significantly lower than that in the model group(P<0.05). CONCLUSION Fufang Longdan compound preparation can promote barrier function recovery after skin laser injury in mice, and its effect is related to inflammation inhibition.