复方感冒灵流浸膏的HPLC指纹图谱及化学成分鉴定

HPLC Fingerprint Analysis and Chemical Component Analysis of Fufang Ganmaoling Fluid Extract

关键词:  复方感冒灵流浸膏  高效液相色谱法  指纹图谱  化学成分  质量控制

Key words:  Fufang Ganmaoling fluid extract  HPLC  fingerprint  chemical component  quality control

摘要:

目的 建立复方感冒灵流浸膏HPLC指纹图谱并对其化学成分进行分析，为复方感冒灵流浸膏质量评价与药效物质基础研究提供依据。方法 采用Kromasil C₁₈色谱柱（4.6 mm×250 mm，5 μm），以乙腈-0.1%甲酸水溶液为流动相，进行梯度洗脱，流速为1.0 mL·min^-1，检测波长290 nm，柱温为30℃，测定10批复方感冒灵流浸膏。利用中药色谱相似度评价系统建立复方感冒灵流浸膏HPLC指纹图谱，并通过对照品对共有峰进行指认，应用HPLC-Q-TOF-MS/MS技术对复方感冒灵流浸膏中的化学成分进行鉴定。结果 建立了复方感冒灵流浸膏成分指纹图谱共有模式，显示12个共有峰，各峰分离度良好，各批次间共有峰相对保留时间RSD均<3.0%，样品间相似度均>0.9。鉴定出复方感冒灵流浸膏中12个化学成分，分别为奎尼酸、原儿茶酸、新绿原酸、swertiajaposide F、绿原酸、隐绿原酸、咖啡酸、二咖啡酰奎宁酸、异绿原酸B、异绿原酸A、异绿原酸C、蒙花苷。结论 该指纹图谱具有良好的精密度、重复性、稳定性，各共有峰间分离度好，可用于复方感冒灵流浸膏的质量评价；该研究为复方感冒灵流浸膏物质基础的研究提供基础。

Abstract:

OBJECTIVE To establish the HPLC fingerprint of Fufang Ganmaoling fluid extract and analyze its chemical components for its quality control and medicinal substance study. METHODS Kromasil C₁₈ column(4.6 mm×250 mm, 5 μm) was used with acetonitrile-0.1% formic acid solution as the mobile phase for gradient elution, and the flow rate was 1.0 mL·min^-1. The detection wavelength was 290 nm, the column temperature was set at 30℃. Ten batches of Fufang ganmaoling fluid extract were detected, their HPLC fingerprint was established by using Similarity Evaluation System for Chromatographic Fingerprint, and the chemical components in common peaks were identified by reference standard and HPLC-Q-TOF-MS/MS. RESULTS Fingerprints of Fufang Ganmaoling fluid extract were established and 12 common peaks were identified. The peaks were well separated, and the relative retention time of common peaks in each batch was less than 3.0% respectively; the similarity was >0.9 among different samples and 12 chemical components were identified as follows:quinic acid, protocatechuic acid, neochlorogenic acid, swertiajaposide F, chlorogenic acid, cryptochlorogenic acid, caffeic acid, dicaffeoylquinic acid, 3,4-dicaffeoylquinic acid, 3,5-dicaffeoylquinic acid, 4,5-dicaffeoylquinic acid and buddleoside. CONCLUSION The method can be used for the quality control of Fufang Ganmaoling fluid extract with good precision, reproducibility and stability, providing basis for further studies on therapeutic efficacy of Fufang Ganmaoling fluid extract.