HPLC测定三棱入血成分含量

Determination of Components of Sparganii Rhizoma Absorbed into Blood by HPLC

关键词:  三棱  入血成分  高效液相色谱法  含量测定

Key words:  sparganii Rhizoma  components absorbed into blood  HPLC  content determination

摘要:

目的 建立同时测定三棱入血成分对羟基苯甲酸、香草酸、阿魏酸和芦丁含量的方法。方法 采用高效液相色谱法，Agilent Eclipse XDB-C₁₈色谱柱（250 mm×4.6 mm，5 μm）；流动相A为0.1%磷酸水溶液，B为甲醇，进行梯度洗脱；流速1.00 mL·min^-1；检测波长254 nm；柱温为30℃。结果 4种成分分离度良好，且均在质量浓度0.000 5~0.25 mg·mL^-1内有良好的线性关系。各组含药血清样本的精密度RSD<10%，方法回收率>；90%；各组样品稳定性RSD均<10%。表明本实验精密度良好，回收率和稳定性均符合药动学要求。结论 该检测方法能够同时、快速、准确测定三棱4种有效成分在血清中的含量。

Abstract:

OBJECTIVE To establish a method for simultaneous determination of phydroxybenzoic acid, vanillic acid, ferulic acid and rutin absorbed into blood of sparganii Rhizoma. METHODS The analysis was carried out by HPLC, chromatographic column:Agilent Eclipse XDB-C₁₈(250 mm×4.6 mm, 5 μm); the mobile phase A was 0.1% phosphoric acid aqueous and solution B was methanol, and the gradient elution HPLC method was used; the flow rate was 1.00 mL·min^-1; the detection wavelength was 254 nm; the column temperature was 30℃. RESULTS The four components showed good separation and good linear relationship in the concentration range of 0.000 5-0.25 mg·mL^-1. The precision of serum sample in each group was good(RSD<10%), the recovery rate was >90%, and the stability of each group was good(RSD<10%), which satisfied the requirements of pharmacokinetics. CONCLUSION The method can determine the four active contents of sparganii Rhizoma in serum simultaneously, accurately and rapidly.