灯盏花乙素对非小细胞肺癌细胞JAK/STAT信号通路的影响

Effects of Scutellarin on JAK/STAT Signal Pathway in Non-small Cell Lung Cancer Cells

关键词:  非小细胞肺癌  灯盏花乙素  JAK2  STAT3  凋亡  Bcl-2

Key words:  non-small cell lung cancer  scutellarin  JAK2  STAT3  apoptosis  Bcl-2

摘要:

目的 研究灯盏花乙素对非小细胞肺癌细胞JAK/STAT信号通路及其凋亡机制的影响。方法 培养非小细胞肺癌A549细胞，经不同浓度灯盏花乙素处理24 h后收集细胞。采用MTT法观察细胞增殖，流式细胞术检测细胞凋亡；RT-qPCR法检测JAK2，STAT3，Pim1和Bcl-2的mRNA表达情况；Western blot检测JAK2和STAT3蛋白表达水平。结果 MTT法和流式细胞术结果显示，灯盏花乙素能降低A549细胞的存活率，促进其凋亡，并呈剂量依赖效应；RT-qPCR和Western blot结果显示，与对照组比较，灯盏花乙素各组JAK2，STAT3，Pim1和Bcl-2的mRNA表达降低，JAK2和STAT3蛋白水平下降，且与加入的灯盏花乙素浓度成反比。结论 灯盏花乙素可以促进非小细胞肺癌A549细胞凋亡，其机制可能是通过抑制JAK/STAT信号转导通路的激活，减少JAK2和STAT3蛋白的表达。

Abstract:

OBJECTIVE To investigate the effect of scutellarin on JAK/STAT signaling pathway and its apoptotic mechanism in non-small cell lung cancer cells. METHODS Non-small cell lung cancer A549 cells were cultured and collected cells after treatment with different concentrations of scutellarin for 24 h. Cell proliferation was analyzed by MTT method, apoptosis was evaluated by flow cytometry. The mRNA expression of JAK2, STAT3, Pim1 and Bcl-2 were detected by RT-PCR; JAK2 and STAT3 protein were detected by Western blot. RESULTS MTT assay and flow cytometry showed that scutellarin reduced the survival rate of A549 cells and promoted its apoptosis in a dose-dependent manner. RT-qPCR and Western blot showed that compared with the control group, the mRNA expression of JAK2, STAT3, Pim1 and Bcl-2 were decreased in the scutellarin group, JAK2 and STAT3 protein were decreased, which were inversely proportional to the concentration of scutellarin. CONCLUSION Scutellarin can promote apoptosis of non-small cell lung cancer A549 cells, which may be through inhibition of JAK/STAT signaling pathway activation and reduction of JAK2 and STAT3 protein expression.