一测多评法同时测定金银花标准汤剂中7种成分的含量

Determination of Seven Components in Lonicerae Japonicae Flos Standard Decoction by QAMS

关键词:  金银花  标准汤剂  一测多评法  相对校正因子

Key words:  Lonicerae japonicae flos  standard decoction  QAMS  relative calibration factor

摘要:

目的 采用一测多评法同时测定金银花标准汤剂中7种成分（新绿原酸、绿原酸、隐绿原酸、木犀草苷、异绿原酸B、异绿原酸A及异绿原酸C）的含量。方法 采用Agilent Eclipse XDB-C₁₈色谱柱（250 mm×4.6 mm，5 μm）进行HPLC测定，以乙腈-0.1%乙酸水溶液为流动相进行梯度洗脱，流速为1.0 mL·min^-1，柱温35℃，检测波长为327 nm。以绿原酸为内标物，建立与其余6种成分的相对校正因子，实现一测多评，考察校正因子的重现性。结果 一测多评法测得的金银花标准汤剂中7种成分含量，与外标法测定结果相比较，2组数据无显著性差异。结论 所建立的一测多评法方便、准确、重现性好，可用于金银花标准汤剂的质量评价。

Abstract:

OBJECTIVE To determine 7 components(neochlorogenic acid, chlorogenic acid, cryptochlorogenic acid, luteoloside, isochlorogenic acid B, isochlorogenic acid A and isochlorogenic acid C) in Lonicerae japonicae flos standard decoction simultaneously by quantitative analysis of multi-components by single-marker(QAMS). METHODS HPLC analysis was performed on an Agilent Eclipse XDB-C₁₈ column(250 mm×4.6 mm, 5 μm) with a mobile phase consisted of acetonitrile-0.1% acetic acid solution in gradient elution manner at a flow rate of 1.0 mL·min^-1. The column temperature was maintained at 35℃, the detection wavelength was set at 327 nm. Chlorogenic acid was used as the internal reference substance, the relative calibration factors(RCFs) of other 6 components were calculated respectively. The validity of the QAMS method was evaluated to realize QAMS. At the same time, the durability of each RCF was investigated. RESULTS No significant differences were found among the quantitative results of 7 components in Lonicerae japonicae flos standard decoction determined by QAMS and external standard method. CONCLUSION The QAMS method is simple, accurate and reproducible, and can be used to evaluate the quality of Lonicerae japonicae flos standard decoction.