姜黄素对宫颈癌小鼠的抗肿瘤活性及免疫功能的影响

Effect of Curcumin on Anti-tumor Activity and Immune Function in Cervical Cancer Mice

关键词:  姜黄素  宫颈癌  抗肿瘤活性  免疫功能

Key words:  curcumin  cervical cancer  anti-tumor activity  immune function

摘要:

目的 探讨姜黄素对宫颈癌小鼠的抗肿瘤活性及免疫功能的影响。方法 采用腹腔及前肢腋下接种U14细胞建立宫颈癌小鼠模型，随机分为模型组、姜黄素组和阳性对照组，每组10只。模型组给予0.9% NaCl 0.2 mL，姜黄素组给予100 mg·mL^-1的姜黄素0.2 mL，连续灌胃14 d，阳性对照组给予顺铂3 mg·kg^-1·d^-1，每隔3 d腹腔注射1次，共5次。比较各组移植瘤形态，检测各组肿瘤体积和重量并计算抑瘤率，ELISA法检测血清中白细胞介素-2（IL-2）、肿瘤坏死因子（TNF-α）、干扰素-γ（IFN-γ）、骨桥蛋白（OPN）、癌胚抗原（CEA）和鳞状细胞癌抗原（SCC-Ag）水平，流式细胞仪检测CD3⁺、CD4⁺、CD8⁺细胞及NKT细胞含量。结果 模型组肿瘤细胞核大、深染，核质增加，核分裂多，姜黄素组和阳性对照组肿瘤细胞明显皱缩，核固缩或碎裂，病理性核分裂减少。姜黄素组和阳性对照组肿瘤体积、质量、IL-2、TNF-α、IFN-γ、OPN、CEA、SCC-Ag、CD8⁺水平显著低于模型组，而抑瘤率、CD3⁺、CD4⁺和NKT细胞含量显著高于模型组，差异均有统计学意义（P<0.05）。结论 姜黄素可抑制宫颈癌小鼠的抗肿瘤活性，其机制可能为增强免疫功能，降低炎症因子和肿瘤标志物水平。

Abstract:

OBJECTIVE To investigate the effects of curcumin on anti-tumor activity and immune function in cervical cancer mice. METHODS Cervical cancer mice model were established by U14 cells inoculated into abdominal cavity and forelimb axillary. The model mice were randomly divided into model group, curcumin group and positive control group, with 10 mice in each group. The model group was given 0.2 mL 0.9% NaCl, the curcumin group was given 100 mg·mL^-1 curcumin, continuous intragastric administration for 14 d, and the positive control group was given 0.2 mL 3 mg·kg^-1·d^-1 cisplatin, once every 3 d, for five times. The morphology of transplanted tumor was compared. The tumor volume and weight were measured and the tumor inhibition rate was calculated. The serum levels of IL-2, TNF-α, IFN-γ, OPN, CEA and SCC-Ag were detected by ELISA. The content of CD3⁺, CD4⁺, CD8⁺ and NKT cells were detected by flow cytometry. RESULTS In the model group, the nuclei were large and hyperchromatic, nucleoplasm increased, and mitosis multiple division. In curcumin group and positive control group, cancer shrunk, nuclei coagulated or fragmented, and pathological mitosis reduced. The levels of tumor volume, weight, IL-2, TNF-a, IFN-γ, OPN, CEA, SCC-Ag and CD8⁺ in curcumin group and positive control group were significantly lower than the model group, and the tumor inhibition rate, CD3⁺, CD4⁺ and NKT cell content were significantly higher than the model group, the differences were statistically significant (P<0.05). CONCLUSION Curcumin can inhibit the anti-tumor activity of cervical cancer mice, the mechanism may be related to the reduction of inflammatory factors and tumor markers by enhancing immune function.