丹参酮ⅡA对酸浆苦素D在大鼠体内药动学特性的影响

Effect of TanshinoneⅡA on Pharmacokinetic Characteristics of Physalin D in Rats

关键词:  丹参酮ⅡA  酸浆苦素D  药动学  羧酸酯酶  相互作用  液质联用

Key words:  tanshinone ⅡA  physalin D  pharmacokinetic  carboxylesterase  interaction  LC-MS/MS

摘要:

目的 研究丹参酮ⅡA对酸浆苦素D在大鼠体内药动学特性的影响。方法 12只SD大鼠随机分为对照组和丹参酮ⅡA组，分别灌胃生理盐水与丹参酮ⅡA，丹参酮ⅡA（50 mg·kg^-1，每天1次）连续给药3 d后，酸浆苦素D灌胃给药后按时间点连续采样，采用HPLC-MS/MS测定酸浆苦素D在大鼠血浆中的含量，采用WinNolin 6.3软件计算主要药动学参数，2组间药动学参数采用SPSS 11.0统计软件对药动学参数进行t检验。结果 对照组和丹参酮ⅡA组酸浆苦素D的药动学参数间差异存在显著性差异（P<0.05），连续给药丹参酮ⅡA 3 d后给酸浆苦素D，酸浆苦素D的AUC_{0-24 h}及C_max均有显著提高。结论 丹参酮ⅡA能够显著影响酸浆苦素D在大鼠体内药动学过程。

Abstract:

OBJECTIVE To study the effect of carboxylesterase inhibitor(tanshinoneⅡA) on pharmacokinetic characteristics physalin D in rats. METHODS Twelve SD rats were randomly divided into the control group and tanshinone Ⅱ A group, which was administrated respectively with normal saline and tanshinone ⅡA. After pretreated with tanshinoneⅡA (50 mg·kg^-1, qd) for 3 d, physalin D was given to the rats by intragastric administration. The plasma concentrations of physalin D were determined by HPLC-MS/MS. The main pharmacokinetic parameters were calculated by WinNonlin 6.3 software, pharmacokinetic parameters between two groups were then compared with t test by SPSS 11.0 software. RESULTS There had significant differences between the two groups in the main pharmacokinetic parameters of physalin D(P<0.05). AUC_{0-24 h} and C_max of oxymatrine D were significantly increased after continuous administration of tanshinone ⅡA for 3 d. CONCLUSION Ginsenone ⅡA can significantly influence the pharmacokinetic process of physalin D in rats.