北青龙衣对大鼠尿液中5种毒性标志物含量的影响

Effect of Juglans Mandshurica on the Contents of Five Toxicity Markers in Rat Urine

关键词:  北青龙衣  毒性标志物  尿液  多反应监测  超高效液相色谱-质谱法

Key words:  Juglans mandshurica  toxicity markers  urine  MRM  UPLC-MS/MS

摘要:

目的 考察北青龙衣鲜品对大鼠尿液中5种毒性代谢物[L-左旋多巴（laevodopa，L-Dopa）、L-酪氨酸（L-tyrosine，L-Tyr）、L-苯丙氨酸（L-phenylalanine，L-Phe）、吲哚乙酸（indoleacetic acid，Int）及尿黑酸（homogentisic acid，HGA）]含量的影响。方法 Wistar大鼠灌胃给予北青龙衣鲜品，周期为1个月，采用MRM方法，建立同时检测5种代谢物的最优方法，并应用Masslynx软件外标法，对各代谢物精准定量。结果 L-Dopa、L-Tyr、L-Phe、Int及HGA的线性范围分别为30~32 000，25~30 000，7.5~8 000，50~10 000，100~10 000 ng·mL^-1，线性良好；与空白组健康大鼠相比，北青龙衣鲜品组尿液中的5种代谢物含量均有所升高。结论 北青龙衣鲜品可影响大鼠体内5种代谢物的正常代谢。

Abstract:

OBJECTIVE To investigate the effects of Juglans mandshurica fresh products on five toxic metabolites, including laevodopa acid(L-Dopa), L-tyrosine(L-Tyr), L-phenylalanine(L-Phe), indoleacetic(Int) and homogentisic acid(HGA), in rat urine. METHODS Wistar rats were given a fresh product of Juglans mandshurica with a period of one month. The MRM method was used to establish the optimal method for simultaneous detection of five metabolites, and the Masslynx software external standard method was used to accurately quantify each metabolite. RESULTS The linear ranges of L-Dopa, L-Tyr, L-Phe, Int, and HGA were 30-32 000, 25-30 000, 7.5-8 000, 50-10 000, 100-10 000 ng·mL^-1, respectively, the linearity was good. Compared with the blank group, the content of the five metabolites in the Juglans mandshurica fresh product groups increased. CONCLUSION Juglans mandshurica fresh products can affect the normal metabolism of five metabolites in rats.