胶艾汤活性成分治疗子宫疾病的网络药理学研究

Study on Network Pharmacology of the Main Active Ingredients of Jiao-ai Tang for the treatment of Uterine Diseases

关键词:  胶艾汤  网络药理学  子宫疾病  靶点  小鼠

Key words:  Jiao-ai Tang  network pharmacology  uterine diseases  target  mice

摘要:

目的 采用网络药理学研究方法探究胶艾汤的药理作用机制。方法 依托中药系统药理学分析平台（TCMSP），检索胶艾汤7味中药的活性成分和基因靶点。采用Cytoscape构建化合物-靶点网络、化合物靶点-疾病靶点和靶点-通路网络，根据在线STRING数据库构建蛋白质相互作用网络，研究胶艾汤药理机制。结果 通过筛选获得64个化合物，化合物-疾病相应关键靶点32个。蛋白质相互作用网络包含20个靶点，关键靶点涉及Ptgs2、F2、Tnf、Il6、F3等。基因本体条目61个，其中生物过程相关的条目34个，细胞组成相关条目20个，分子功能相关的条目7个。KEGG通路富集分析通路4条。结论 本研究初步验证了胶艾汤的基本药理作用及其机制，为进一步探究其药理作用奠定了基础。

Abstract:

OBJECTIVE To explore the pharmacological mechanism of Jiao-ai Tang by means of network pharmacology. METHODS The active components and gene targets of 7 traditional Chinese medicines were retrieved on the basis of TCMSP. Cytoscape was used to construct compound-target network, compound-target-disease target and target-pathway network, and protein-protein interaction network was constructed according to online STRING database to study the pharmacological mechanism of Jiao-ai Tang. RESULTS sixty-four compounds were obtained through screening, and 32 corresponding key targets were obtained. Protein-protein interaction network contained 20 targets, key targets including Ptgs2, F2, Tnf, Il6, F3, etc. There were 61 items of gene ontology, including 34 items related to biological process, 20 items related to cell composition and 7 items related to molecular function. KEGG channel enrichment analysis of 4 pathways. CONCLUSION This study preliminarily verified the basic pharmacological effect and mechanism of Jiao-ai Tang, and laid a foundation for further exploring its pharmacological effect.