桑黄水煎液对D-半乳糖衰老模型小鼠的抗氧化作用

Effect of Sanghuang decoction on oxidative damage induced by D-galactose in mice

关键词:  桑黄  抗氧化  D-半乳糖  氧化损伤

Key words:  Inonotus sanghuang  antioxidant  D-galactose  oxidative damage

摘要:

目的 观察桑黄水煎液对D-半乳糖诱导小鼠氧化损伤的保护作用，并探讨其保护作用的可能机制。方法 ①ICR小鼠分为正常对照组，桑黄水煎液低、中、高剂量（2，6，12 g·kg^-1）组，连续给药15 d。②ICR小鼠分为正常对照组、模型组、维生素E组（50 mg·kg^-1），桑黄水煎液低、中、高剂量（2，6，12 g·kg^-1）组，小鼠颈背部皮下注射D-半乳糖（120 mg·kg^-1）造成亚急性衰老模型，按分组连续给药42 d。比色法测定小鼠肝脏及血清中丙二醛（MDA）含量和抗氧化酶活力，如超氧化合物歧化酶（SOD）、总抗氧化能力（T-AOC）、过氧化酶（POD）等。Western blot检测肝脏中核因子E2相关因子2（Nrf2）和血红素氧合酶1（HO-1）的表达。结果 桑黄水煎液对正常小鼠和D-半乳糖诱导小鼠体质量及脏器指数均无明显影响。桑黄水煎液可显著提高正常小鼠肝脏中T-AOC和SOD酶活力（P<0.01，P<0.05）。各剂量桑黄水煎液可显著提高衰老模型小鼠血清中T-AOC，降低MDA含量（P<0.01，P<0.05），同时中剂量增强肝脏中SOD （P<0.05）和POD酶活力（P<0.01），中、高剂量均上调肝脏中Nrf2和HO-1蛋白表达水平（P<0.01）。结论 桑黄水煎液具有提高正常小鼠抗氧化能力和改善D-半乳糖致小鼠氧化损伤作用，此作用可能是通过调节Nrf2/HO-1途径增加体内抗氧化酶的活性，减少过氧化脂质的生成，进而提高机体抗氧化能力。

Abstract:

OBJECTIVE To observe the protective effect of Sanghuang decoction on oxidative damage induced by D-galactose in mice and explore the possible mechanism. METHODS ①The mice were divided into the normal group, the Sanghuang decoction low, middle, high dose(2, 6, 12 g·kg^-1) group, and administrated for 15 days. ②Mice were divided into the normal group, model group, vitamin E (50 mg·kg^-1) group, Sanghuang decoction low, middle, high dose(2, 6, 12 g·kg^-1) group. Subacute aging model was induced by subcutaneous injection of D-galactose(120 mg·kg^-1) into the neck and back of mice. The mice were continuous administrated for 42 d. The content of malondialdehyde(MDA) and activity of antioxidant enzymes such as superoxide dismutase(SOD), total antioxidant capacity(T-AOC) and peroxidase(POD) were measured by colorimetry in the liver and serum of mice. The levels of nuclear factor E2-related factor 2(Nrf2) and heme oxygenase 1(HO-1) proteins in liver were detected by Western blot. RESULTS Sanghuang decoction had no significant effect on body weight and organ index of normal mice and D-galactose induced mice. Sanghuang decoction could significantly improve the activity of T-AOC and SOD in the liver of normal mice(P<0.01, P<0.05). Sanghuang decoction could significantly increase T-AOC and reduce MDA content in serum of mice with aging model(P<0.01, P<0.05), meanwhile middle dose enhanced the activities of SOD(P<0.05) and POD(P<0.01), and middle and high dose upregulated the levels of Nrf2 and HO-1 proteins in liver of mice with aging model(P<0.01, P<0.05). CONCLUSION Sanghuang decoction can enhance the antioxidant capacity of normal mice and improve the oxidative damage induced by D-galactose, which maybe increase the activity of antioxidant enzymes in vivo, reduce the generation of lipid peroxide and improve the antioxidant capacity via Nrf2/HO-1 pathway.