如意珍宝丸对慢性神经源性疼痛大鼠炎症因子水平的影响

Effect of Ruyi Zhenbao Pill on the Level of Inflammatory Cytokines in Rats with Chronic Neurogenic Pain

关键词:  如意珍宝丸  慢性坐骨神经缩窄损伤模型  肿瘤坏死因子α  白介素10

Key words:  Ruyi Zhenbao pill  sciatic nerve chronic constriction injury  TNF-α  IL-10

摘要:

目的 观察如意珍宝丸对坐骨神经慢性缩窄损伤（chronic constriction injury，CCI）模型大鼠慢性神经病理性疼痛的保护作用及炎症因子水平的影响。方法 经热缩腿反射潜伏期（thermal withdrawal latency，TWL）检测合格的雄性SD大鼠，麻醉后手术分离坐骨神经主干，4-0含铬肠线环绕结扎坐骨神经三支分叉近端4次，每结间隔约1 mm，复制慢性神经病理性疼痛模型，选择建模成功的大鼠45只，随机分为5组，每组9只，分别为模型组，如意珍宝丸低（0.5g·kg^-1）、中（1.5 g·kg^-1）、高剂量组（4.5 g·kg^-1），罗通定组（18 mg·kg^-1），并设置假手术对照组，按15 mL·kg^-1给药体积，每日1次，连续灌胃给药10 d。在第5，7，10天给药30 min后测定TWL。末次给药次日，腹主动脉采血，检测细胞因子水平。结果 与模型组比较，给药10 d后如意珍宝丸低、中、高剂量组，罗通定组大鼠TWL明显延长，血清IL-10含量明显降低；如意珍宝丸高剂量组、罗通定组大鼠血清中TNF-α含量明显降低。结论 如意珍宝丸可能通过调节炎症因子水平，减轻疼痛炎症级联反应，抑制中枢神经敏化，而对坐骨神经CCI大鼠慢性神经源疼痛起到一定的保护作用。

Abstract:

OBJECTIVE To observe the protective effect of Ruyi Zhenbao pill on rat with sciatic nerve chronic constriction injury(CCI) and explore the effect on cytokine levels. METHODS Eligible male SD rats were screened by thermal withdraw latency(TWL). The sciatic nerve trunk was surgically isolated after anesthesia. The proximal end of the three branches of sciatic nerve was ligated by 4-0 chromium-containing enteric line for four times, with the interval of about 1 mm between each node, to replicate the model of chronic neuropathic pain. The 45 rats with successful modeling were randomly divided into 5 groups, 9 in each group, the groups were model control group, Ruyi Zhenbao pills low(0.5 g·kg^-1), middle(1.5 g·kg^-1), high(4.5 g·kg^-1) dose group, rotundine group(18 mg·kg^-1). And set up a sham-operated control group. The corresponding drug or solvent was administered by intragastric once a day for 10 consecutive days. TWL was measured 30 min after administration on days 5, 7, and 10. On the 11th day, blood was collected from the abdominal aorta and cytokine levels were measured. RESULTS TWL of rats in Ruyi Zhenbao pill low, middle, high dose group and rotundine group was obviously prolonged, and the content of IL-10 was obviously decreased. The content of TNF-α in the serum of rats in Ruyi Zhenbao pill high dose group and rotundine group was obviously decreased. CONCLUSION Ruyi Zhenbao pill may regulate the level of cytokines, alleviate the pain inflammatory cascade, inhibit central nervous system sensitization, and play a protective role in the chronic neuropathic pain caused by sciatic nerve CCI.