利节通痹酒剂对胶原诱导性关节炎大鼠的影响

Effects of Lijie Tongbi Liquor on Collagen-Induced Arthritis Rats

关键词:  胶原诱导性关节炎  利节通痹酒剂  抗炎  镇痛

Key words:  collagen-induced arthritis  Lijie Tongbi liquor  anti-inflammatory  analgesic

摘要:

目的 考察利节通痹酒剂（Lijie Tongbi liquor，LJTB）对胶原诱导性关节炎（collagen-induced arthritis，CIA）模型大鼠的抗炎镇痛作用以及对关节损伤的影响。方法 通过足跖注射牛Ⅱ型胶原建立大鼠CIA模型。40只造模成功的SD大鼠随机分为模型组、雷公藤多苷组（阳性对照组）以及LJTB 15.5，7.75 g·kg^-1组，并设正常对照组。连续灌胃给药4周，定期考察一般行为学、关节炎指数（arthritis index，AI）评分、足肿胀度、痛阈值，同时检测肝功能[谷丙转氨酶（ALT）、谷草转氨酶（AST）]和肾功能[肌酐（Cr）、尿素氮（BUN）]；给药结束后，通过酶联免疫法检测血清C反应蛋白（C-reactive protein，CRP）及类风湿因子（rheumatoid factor，RF）水平，并观察踝关节病理组织学变化。结果 与模型组比较，连续给药3周，LJTB能显著降低胶原诱导性关节炎大鼠的AI评分（P<0.05或P<0.01），并改善足肿胀度。连续给药4周，LJTB 15.5 g·kg^-1显著降低大鼠足肿胀度、AI评分、血清CRP及RF含量（P<0.05或P<0.01），显著升高痛阈值（P<0.05），减少关节软骨损伤。给药期间，LJTB 15.5，7.75 g·kg^-1对ALT、AST及Cr、BUN均无显著影响。结论 LJTB通过降低CRP水平，调节炎症反应，缓解关节软骨损伤；升高痛阈值，产生镇痛作用，且无肝肾毒性。

Abstract:

OBJECTIVE To investigate the anti-inflammatory and analgesic effects of Lijie Tongbi liquor(LJTB) on collagen-induced arthritis(CIA) rats and the effects on joint damage. METHODS CIA rats' model was established by injection of Bovine Type Ⅱ Collagen. Forty CIA rats were grouped into 4 groups, LJTB two different dose groups(LJTB 15.5, 7.75 g·kg^-1), model group and Tripterygium glycosides group(positive group) respectively. And another 10 normal rats were grouped as normal control. The drug was given orally for 4 weeks. General behavior of rats, arthritis index(AI), foot swelling degree, pain threshold, liver function indexes(ALT, AST) and renal function indexes(Cr, BUN) were measured at regular intervals. After administration, serum C-reactive protein(CRP) and rheumatoid factor(RF) levels were detected in enzyme-linked immunosorbent assay, and joints' pathological damage situations were studied by HE staining of joint sections. RESULTS After 3 weeks of administration, compared with model group, LJTB can significantly decreased foot swelling degree and AI(P<0.05 or P<0.01). And after 4 weeks' continuous administration, foot swelling degree, AI, serum CRP and RF levels were significantly decreased in LJTB 15.5 g·kg^-1 group(P<0.05 or P<0.01), and pain threshold were significantly increased(P<0.05), at the same time, joint injury of which were eased. LJTB 15.5 and 7.75 g·kg^-1 didn't play any significantly impact on liver and kidney functions(ALT, AST, Cr and BUN) of rats. CONCLUSION LJTB can regulate inflammatory of rats and relieves joint cartilage damage via decreased CRP. Also, pain threshold of rats was significantly increased without liver and kidney toxicity.