HPLC测定绞股蓝中七叶胆苷XLVI和人参皂苷Rb3含量

Determination of Gypenoside XLVI and Ginsenoside Rb3 in Gynostemma pentaphyllum by HPLC

关键词:  绞股蓝  七叶胆苷XLVI  人参皂苷Rb₃

Key words:  Gynostemma pentaphyllum (Thunb.) Mak.  gypenoside XLVI  ginsenoside Rb₃

摘要:

目的 建立HPLC测定绞股蓝中七叶胆苷XLVI和人参皂苷Rb₃含量的方法。方法 采用Ultimate XB-C₁₈色谱柱（250 mm×4.6 mm，5 μm），以乙腈（A）-水（B）线性梯度洗脱，检测波长203 nm，流速1 mL·min^-1，柱温30℃。结果 七叶胆苷XLVI在3.30~39.62 μg·mL^-1范围内线性良好（r²=0.999 7），平均回收率为113%，RSD为1.84%；人参皂苷Rb₃在3.27~39.26 μg·mL^-1范围内线性良好（r²=0.999 7），平均回收率为102%，RSD为2.82%。结论 该方法有较好的分离效果，准确性、精密度和重复性良好，为绞股蓝的研究和质量控制提供了科学依据。

Abstract:

OBJECTIVE To establish a reversed phase HPLC for simultaneous determination of gypenoside XLVI and ginsenoside Rb₃ in Gynostemma pentaphyllum. METHODS The separation of gypenoside XLVI and ginsenoside Rb₃ were performed on a Ultimate XB-C₁₈ (250 mm×4.6 mm, 5 μm) with methanol(A)-water(B) as mobile phase in a gradient elution program at a flow rate of 1 mL·min^-1. The column temperature was maintained at 30℃. The detection wavelength was 203 nm. RESULTS Gypenoside XLVI and ginsenoside Rb₃ showed a good linearity range in 3.30-39.62 μg·mL^-1 (r²=0.999 7) and 3.27-39.26 μg·mL^-1 (r²=0.999 7), respectively. The average recoveries were 113% and 102%, RSD were 1.84% and 2.82%, respectively. CONCLUSION This method is accurate with high simplify, stability, and reliability, and can be used for quality research of Gynostemma pentaphyllum.