CO2超临界流体色谱法同时测定莪术油中3个倍半萜类成分的含量

Simultaneous Determination of Three Sesquiterpenoids in the Zedoary Turmeric Oil by CO2-Supercritical Fluid Chromatography

关键词:  CO₂超临界流体色谱法  呋喃二烯  牻牛儿酮  莪术二酮  莪术油

Key words:  CO₂-supercritical fluid chromatography  furanodiene  curdione  germacrone  Zedoary Turmeric oil

摘要:

目的 建立CO₂超临界流体色谱法测定莪术油中呋喃二烯、牻牛儿酮和莪术二酮含量的方法。方法 采用ACQUITY UPC² HSS C₁₈ SB色谱柱（3.0 mm×150 mm，1.8 μm），以CO₂-乙腈为流动相，梯度洗脱；流速为1.0 mL·min^-1；检测波长为216 nm，柱温为55℃，背压为2 000 psi。结果 呋喃二烯在2.67~1 337.26μg·mL^-1内线性关系良好（r=1.000），加样回收率为97.94%（n=6，RSD=1.50%）。牻牛儿酮在2.77~1 386.00 μg·mL^-1内线性关系良好（r=1.000），加样回收率为96.07%（n=6，RSD=1.68%）；莪术二酮在6.99~3 493.00 μg·mL^-1内线性关系良好（r=1.000），加样回收率为99.33%（n=6，RSD=1.88%）。结论 本方法快捷准确、稳定且绿色环保，可用于莪术油中上述3个倍半萜类成分的质量控制。

Abstract:

OBJECTIVE A CO₂-Supercritical Fluid Chromatography(CO₂-SFC) method was established for the simultaneous determination of furanodiene, curdione and germacrone in the Zedoary Turmeric Oil. METHODS A UPC² HSS C₁₈ SB analytical column(3.0 mm×150 mm, 1.8 μm) was adopted and the mobile phase consisted of CO₂-acetonitrile with linear gradient elution(0-8 min, 99.5%→84% of CO₂) at the flow rate of 1 mL·min^-1. The detection wavelength was 216 nm, the column temperature was maintained at 55℃, and the back pressure was was kept at 2 000 psi. RESULTS The linear range of furanodiene was 2.67-1 337.26 μg·mL^-1 (r=1.000), and the average recovery(n=6) was 97.94% with RSD of 1.50%. The linear range of curdione was 2.77-1 386.00 μg·mL^-1 (r=1.000), and the average recovery(n=6) was 96.07% with RSD of 1.68%. The linear range of germacrone was 6.99-3 493.00 μg·mL^-1 (r=1.000), and the average recovery(n=6) was 99.33% with RSD of 1.88%. CONCLUSION The established method is rapid, accurate, and it can protect environment, which provides a new method for the quality control of furanodiene, curdione and germacrone in the Zedoary Turmeric Oil.