姜黄素水凝胶对牙周炎大鼠的疗效研究

Therapeutic Effect of Curcumin Hydrogel on Rats with Periodontitis

关键词:  姜黄素  牙周炎  水凝胶  肿瘤坏死因子  前列腺素E2

Key words:  curcumin  periodontitis  hydrogels  tumor necrosis factor-α(TNF-α)  prostaglandin E2(PGE2)

摘要:

目的 制备姜黄素水凝胶并评价其对牙周炎大鼠的治疗效果。方法 以姜黄素为主药，羟丙基-β-环糊精（hydroxypropyl-β-cyclodextrin，HP-β-CD）为吸收促进剂，泊洛沙姆为凝胶基质，制备一种新型牙周袋内注射用姜黄素水凝胶，并评价其黏度随温度变化关系。建立大鼠牙周炎模型，以牙龈指数、出血指数、探诊出血、探诊深度、牙槽骨吸收情况、病理切片、前列腺素E2（prostaglandin E2，PGE2）及肿瘤坏死因子（tumor necrosis factor-α，TNF-α）为指标评价姜黄素水凝胶治疗牙周炎的效果。结果 姜黄素水凝胶37℃黏度适于局部给药。牙周炎大鼠经姜黄素水凝胶治疗后，牙周组织炎症程度及牙周破坏程度比模型组和空白凝胶组减轻，牙周附着水平显著降低（P<0.01），牙龈组织中PGE2、TNF-α水平显著降低（P<0.01）。结论 姜黄素水凝胶治疗大鼠牙周炎疗效明显，有望成为一种新型牙周炎治疗制剂。

Abstract:

OBJECTIVE To prepare the curcumin hydrogel and evaluate its therapeutic effect on rats with periodontitis. METHODS The curcumin hydrogel for periodontal pocket injection were prepared with hydroxypropyl-β-cyclodextrin (HP-β-CD) as the absorption enhancer and poloxamer as the gel matrix. The relationship between viscosity and temperature of curcumin hydrogels was evaluated. Rats with periodontitis was established. Gingival index, bleeding index, bleeding on probing, probing depth, alveolar bone resorption, pathological section, prostaglandin E2(PGE2) and tumor necrosis factor-α(TNF-α) were used as the indicators to evaluate the therapeutic effect of curcumin hydrogels.RESULTS The viscosity of curcumin hydrogel was suitable for topical drug delivery. Compared with the model group and the blank hydrogel group, the inflammation degree and periodontal damage of periodontal tissues in the rats of the curcumin hydrogel group was less severe. In addition, periodontal adhesion level significantly decreased(P<0.01), and the levels of PGE2 and TNF-α in gingival tissues also decreased significantly(P<0.01). CONCLUSION The curcumin hydrogel is effective to treat rats periodontitis, and it is expected to become a novel formulation for periodontitis treatment.