CO2超临界流体色谱法建立片姜黄指纹图谱检查方法的研究

Study on the Fingerprint of Wenyujin Rhizoma Concisum by CO2-supercritical Fluid Chromatography

关键词:  CO₂超临界流体色谱法  片姜黄  指纹图谱

Key words:  CO₂-supercritical fluid chromatography  Wenyujin Rhizoma Concisum  fingerprint

摘要:

目的 采用CO₂超临界流体色谱法，建立片姜黄指纹图谱的检查方法。方法 相似度测定采用ACQUITY UPC² HSS C₁₈ SB色谱柱（3.0 mm×150 mm，1.8μm），以CO₂-乙腈为流动相，梯度洗脱；流速为1.0 mL·min^-1；检测波长为216 nm，柱温为55℃，背压为2 000 psi。结果 建立的片姜黄指纹图谱检查方法可用于片姜黄相似度的测定，15批样品的平均相似度为0.968，RSD为2.6%，而测定其他非正品来源的相似度均<0.400。结论 建立的方法快捷、稳定且环境友好，能够较好地区分片姜黄和其他非正品。

Abstract:

OBJECTIVE To develop the fingerprint of Wenyujin Rhizoma Concisum by CO₂-supercritical fluid chromatography(CO₂-SFC). METHODS The determination of similarity was performed on a ACQUITY UPC² HSS C₁₈ SB analytical column(3.0 mm×150 mm, 1.8 μm) with CO₂(A)-acetonitrile(B) as mobile phase in a gradient elution program at a flow rate of 1 mL·min^-1. The detection wavelength was 216 nm. The column temperature was maintained at 55℃. and the back pressure was 2 000 psi. RESULTS the established determination method for the fingerprint of Wenyujin Rhizoma Concisum could be used for the similarity determination on it, the average similarities of the 15 batches was 0.968 with RSD was 2.6%, while the unofficial samples were <0.400. CONCLUSION The developed method is simplify, rapidly, stability, and environmental friendly, which is helpful to identify the official and unofficial samples of Wenyujin Rhizoma Concisum.