土茯苓对环孢素A诱导的免疫抑制小鼠免疫功能的影响

Effect of Smilacis Glabrae Rhizoma on the Immune Function of Immunosuppressive Mice Induced by Cyclosporin A

关键词:  土茯苓  免疫  T细胞  环孢素A

Key words:  Smilacis Glabrae Rhizoma  immune  T cell  cyclosporin A

摘要:

目的 探讨土茯苓水提液对环孢素A诱导的免疫抑制小鼠免疫功能的影响。方法 小鼠腹腔注射环孢素A 5 d致免疫抑制模型，造模成功后随机分为模型组、左旋咪唑组（3.79 g·kg^-1，阳性药）和土茯苓低、中、高剂量组（4.55，9.1，13.65 g·kg^-1）（n=16），连续给药治疗5 d后，处死小鼠，取脾、胸腺，称重计算脾指数和胸腺指数，流式细胞术检测CD3⁺CD4⁺，CD3⁺CD8⁺；酶联免疫吸附法检测IL-2，IL-6，IFN-γ。结果 造模后小鼠脾指数显著高于正常对照组（P<0.05），胸腺指数明显降低，脾T细胞CD3⁺CD4⁺显著降低（P<0.05）；与模型组比较，各治疗组小鼠脾指数均有不同程度降低，土茯苓水提液中剂量组差异显著（P<0.05），但胸腺指数无显著变化；左旋咪唑组、土茯苓中、高剂量组脾T细胞CD3⁺CD4⁺数目显著增加（P<0.01），各治疗组脾脏IFN-γ均显著下降（P<0.05）。结论 土茯苓水提液能通过降低脾指数、增加脾T细胞CD3⁺CD4⁺的数目，增强机体免疫功能，并下调脾脏内较高的IFN-γ，其调节机制可能与左旋咪唑类似。

Abstract:

OBJECTIVE To study the effect of Smilacis Glabrae Rhizoma on immune cells in immunosuppressive mice model. METHODS The model was induced by intraperitoneal injection of cyclosporin A. After 5 d of modeling, the model was successful, modeled mice was randomly divided into model control group, levamisole treatment group(3.79 g·kg^-1, positive control group) and Smilacis Glabrae Rhizoma low, medium, high dose(4.55, 9.1, 13.65 g·kg^-1) treatment group (n=16). After 5 d treatment, mice were sacrificed and tissues were collected, spleen and thymus were weighted, CD3⁺CD4⁺ and CD3⁺CD8⁺ amount were detected by flow cytometry, IL-2, IL-6, IFN-γ were detected by Elisa. RESULTS Compared with normal control group, spleen index increased significantly(P<0.05), thymus index and CD3⁺CD4⁺ amount in spleen(P<0.05) were decreased in model group. compared with the model control group, the spleen index of mice in each treatment group was decreased to varying degrees, and the difference in the Smilacis Glabrae Rhizoma medium treatment group was significant, the CD3⁺CD4⁺ amount in spleen T cells was significantly increased in levamisole group, Smilacis Glabrae Rhizoma medium and high treatment group (P<0.01), then IFN-γ in spleen was significantly decreased in each treatment groups(P<0.05), while the thymus index did not change significantly. CONCLUSION Smilacis Glabrae Rhizoma could enhance the immune function by increasing the number of CD3⁺CD4⁺, decreasing IFN-γ in spleen and the regulate mechanism may be similar way of levamisole.