曲昔派特对大鼠乙酸型慢性胃溃疡调节作用的研究

Study on Regulation Effect of Troxipide on Gastric Ulcer Induced by Acetic Acid in Rats

关键词:  曲昔派特  胃溃疡  乙酸  大鼠

Key words:  troxipide  gastric ulcer  ethanoic acid  rat

摘要:

目的 研究曲昔派特（troxipide，PT）对乙酸型胃溃疡的治疗效果，并探讨其作用机制。方法 采用乙酸灌胃法制备大鼠胃溃疡模型，将造模成功的大鼠随机分为模型组，雷尼替丁阳性组，PT高、低剂量组，每组10只，另取10只健康大鼠作为空白对照组。造模14 d后，连续给药7 d处死所有大鼠，测量溃疡面积，计算溃疡指数与抑制率；HE染色进行组织病理形态学观察，检测胃组织中IL-1、IL-6、NF-κB、TNF-α、PGE2、MDA、SOD活性和氧自由基的相对含量，以及胃组织中EGF、VEGF、TNF-α、NF-κB mRNA的表达。结果 与模型组比较，各给药组均能明显改善溃疡面积和溃疡指数（P<0.05或P<0.01）；胃组织病理变化有不同程度的改善；胃组织中IL-1、IL-6、NF-κB、TNF-α、PGE2、MDA、黏膜氧自由基的相对含量明显降低，SOD的水平明显升高（P<0.05或P<0.01），以及胃组织中EGF、VEGF、TNF-α、NF-κB mRNA的表达水平明显改善（P<0.05或P<0.01）。结论 PT治疗胃溃疡效果较好，其作用机制可能与促进胃溃疡大鼠胃肠黏膜血管新生、上皮和边缘表皮细胞增生分化，并消除炎症因子等作用有关。

Abstract:

OBJECTIVE To investigate the efficacy and mechanism of troxipide on treatment of chronic gastric ulcer induced by acetic acid. METHODS Using chronic acetic acid to establish gastric ulcer model, the successful model of the rats were randomly divided into model group, ranitidine group, high dose and low dose group of troxipide. Each group had 10 rats, another 10 healthy rats were set as a blank control group. After 14 d of model, the drugs were given to rats daily for 7 d by intragastric administration and then executed. The ulcer area of rats in each group were measured, the ulcer index and inhibition rate were calculated. Histopathological morphology was observed by HE, the levels of IL-1, IL-6, NF-κB, TNF-α, PGE2, MDA, SOD activity and the relative content of oxygen free radicals were detected in gastric tissue. The mRNA expression of EGF, VEGF, TNF-α, NF-κB in gastric tissue were examined. RESULTS Compared with the model group, ulcer area and ulcer index were significantly improved(P<0.05 or P<0.01). The pathological changes of gastric tissue were improved in different degree; the levels of IL-1, IL-6, NF-κB, TNF-α, PGE2, MDA activity and the relative content of oxygen free radicals in gastric tissue reduced significantly, and SOD was significantly increased(P<0.05 or P<0.01); the mRNA levels of EGF, VEGF, TNF-α and NF-κB in gastric tissue were significantly improved(P<0.05 or P<0.01). CONCLUSION PT has an effectively effect on gastric ulcer, and its mechanism may be related to promoting the proliferation and differentiation of angiogenesis, epithelial and marginal epidermal cells in the gastrointestinal mucosa of gastric ulcer rats, and the role of eliminating inflammatory factors.