HPLC-荧光法测定酒女贞子中羟基酪醇、红景天苷和酪醇

Determination of Hydroxytyrosol, Salidroside and Tyrosol in Wine-fried Ligustri Lucidi Fructus by HPLC with Fluorescence Detection

关键词:  高效液相色谱法  荧光  羟基酪醇  红景天苷  酪醇  酒女贞子

Key words:  HPLC  Fluorescence detection  hydroxytyrosol  salidroside  tyrosol  wine-fried Ligustri Lucidi Fructus

摘要:

目的 建立一种简单、高灵敏度，可同时测定酒女贞子中羟基酪醇、红景天苷和酪醇含量的HPLC-荧光法。方法 采用Amethyst C₁₈-P柱（250 mm×4.6 mm，5 μm），以水-乙腈（92∶8）为流动相等度洗脱，激发波长为280 nm，发射波长为310 nm，柱温35℃。结果 羟基酪醇、红景天苷和酪醇在一定浓度范围内线性关系良好，检测限分别可达23.0，10.5，9.82 ng·mL^-1。酒女贞子中羟基酪醇、红景天苷和酪醇的含量范围分别为642.9~867.2 μg·g^-1，1 932.3~15 357.5 μg·g^-1，239.1~638.1 μg·g^-1。结论 所建立的HPLC-荧光法灵敏度高，选择性好、准确度高，可用于酒女贞子中羟基酪醇、红景天苷和酪醇的含量测定。

Abstract:

OBJECTIVE To develop a simple and sensitive HPLC with fluorescence detection method for simultaneous determination of hydroxytyrosol, salidroside and tyrosol in wine-fried Ligustri Lucidi Fructus. METHODS The analysis was performed on a Amethyst C₁₈-P column(250 mm×4.6 mm, 5 μm) with isometric elution using water and acetonitrile(92:8). The detection wavelengths were at 280 nm and 310 nm for excitation and emission. The column temperature was 35℃. RESULTS The good linearity was in the certain concentration range, and the limit of quantitation of hydroxytyrosol, salidroside and tyrosol were 23.0, 10.5, 9.82 ng·mL^-1, respectively. The range of content of hydroxytyrosol, salidroside and tyrosol in wine-fried Ligustri Lucidi Fructus were 642.9-867.2 μg·g^-1, 1 932.3-15 357.5 μg·g^-1, 239.1-638.1 μg·g^-1, respectively. CONCLUSION The developed HPLC-Fluorescence detection method is sensitive, selective and accurate for simultaneous determination of hydroxytyrosol, salidroside and tyrosol in wine-fried Ligustri Lucidi Fructus.