Box-Behnken响应面法优化青黛炮制条件

Box-Behnken Response Surface Methodology to Optimize the Processing Conditions of Indigo Naturalis

关键词:  Box-Behnken响应面法  青黛  靛蓝  靛玉红

Key words:  Box-Behnken response surface methodology  Indigo Naturalis  indigo  indirubin

摘要:

目的 利用Box-Behnken响应面法优化青黛炮制参数。方法 分别以靛蓝、靛玉红产率为响应值，在单因素试验基础上，采用响应面试验设计方法优化青黛炮制条件。用Expert Design 8.0.6软件分析数据。结果 由回归模型预测的定向生成靛玉红的最佳条件为物料比1：10，盐酸浓度3%，通O₃时间73 s，提取温度79℃，提取时间33 min；在此条件下得到的靛玉红产率为0.758 mg·g^-1。由回归模型预测的定向生成靛蓝的最佳条件为物料比1：10，盐酸浓度3.4%，提取温度79℃，提取时间33 min；在此条件下得到的靛蓝产率为2.07 mg·g^-1。结论 本实验系统优化了定向生成靛玉红、靛蓝的青黛炮制条件，提高了青黛品质。

Abstract:

OBJECTIVE To optimize the processing parameters of Indigo Naturalis by Box-Behnken response surface methodology. METHODS The yields of indigo and indirubin were taken as the response values respectively, Box-Behnken response surface design method was used to optimize the processing parameters of Indigo Naturalis based on the single factor methodology. Expert Design 8.0.6 software was used to analyze the data. RESULTS The optimal conditions for the orientation-generated indirubin predicted by the regression model were as follows:the material ratio was 1:10, the hydrochloric acid concentration was 3%, the O₃ time was 73 s, the extraction temperature was 79℃, the extraction time was 33 min, and the yield of the indirubin obtained under this condition was 0.758 mg·g^-1. The optimal conditions for the orientation-generated indigo predicted by the regression model were as follows:the material ratio was 1:10, the hydrochloric acid concentration was 3.4%, the extraction temperature was 79℃, and the extraction time was 33 min. The yield of indigo obtained under this condition was 2.07 mg·g^-1. CONCLUSION The study optimizes the processing parameters of the orientation-generated indirubin and indigo, and improves the quality of Indigo Naturalis.