离子色谱法测定小儿复方氨基酸注射液中的氨含量

Determination of ammonium in pediatric compound aminoacid injection by Ion Chromatograpy

关键词:  离子色谱法  阳离子交换柱  电导检测器  小儿复方氨基酸注射液  氨  含量测定

Key words:  ion chromatography  cation exchange column  conductivity detector  pediatric compound aminoacid injection  ammonium  content determination

摘要:

目的 采用离子色谱法优化测定小儿复方氨基酸注射液中的氨含量。方法 采用阳离子交换色谱柱RFICTM IonPac^® CG16（4 mm×50 mm），保护柱CS16（4 mm×250 mm）；以20 mmol·L^-1甲基磺酸为流动相A，以100 mmol·L^-1甲基磺酸为流动相B，梯度洗脱，流速为1.0 mL·min^-1；检测器为电导检测器（带抑制器CERS 300，4 mm）；进样体积25 μl。结果 氨的线性范围为0.149 9~5.997 9 μg·ml^-1（r=0.998 4），平均回收率为96.8%，RSD为2.82%。结论 该法专属性高、结果准确可靠，可用于小儿复方氨基酸注射液中氨的含量控制。

Abstract:

OBJECTIVE To use ion chromatography method to optimize the assay of ammonium in pediatric compound aminoacid injection. METHODS The IC method was performed on a cation exchange column RFICTM IonPac® CG16 (4 mm×50 mm), CS16(4 mm×250 mm) by gradient elution at a flow rate of 1.0 mL·min^-1. The mobile phase A was 20 mmol·L^-1 methanesulfonic acid, the mobile phase B was 100 mmol·L^-1 methanesulfonic acid. The detector was conductivity detector with a suppressor(CERS 300, 4 mm) and the injection volume was 25 μl. RESULTS The linear range of the ammonium was 0.149 9-5.997 9 μg·mL^-1(r=0.998 4). The average recovery of it was 96.8% and the RSD was 2.82%. CONCLUSION The method was specific, accurate and suitable for the assay of ammonium for quantitative control of pediatric compound aminoacid injection.