美洲大蠊提取物对博来霉素诱导大鼠肺纤维化的疗效研究

Effect of Periplaneta Americana Extract on Bleomycin-induced Pulmonary Fibrosis in Rats

关键词:  美洲大蠊  活性提取物  肺纤维化  博来霉素

Key words:  Periplaneta Americana  active extract  pulmonary fibrosis  bleomycin

摘要:

目的 探讨美洲大蠊抗肺纤维化活性提取物（ML-HB）对博来霉素诱导肺纤维化大鼠的保护作用。方法 将SD雄性大鼠随机分为正常组、模型组、阳性组（地塞米松3 mg·kg^-1，qd）及ML-HB高、中、低剂量组（120，60，30 mg·kg^-1，qd），采用气管暴露式注入博来霉素（5 mg·kg^-1）制备大鼠肺纤维化模型，造模24 h后经腹腔给药干预。给药第30天处死大鼠，测定肺指数，ELISA法检测肺匀浆中肿瘤坏死因子-α（tumor necrosis factor-α，TNF-α）和转化生长因子-β1（transforming growth factor-β1，TGF-β1）的表达，紫外法检测羟脯氨酸（hydroxyproline，HYP）的表达，HE染色观察肺组织炎症病变，Masson染色判定肺纤维化程度，免疫组化法观察α-平滑肌肌动蛋白（α-smooth muscleactin，α-SMA）与I型胶原蛋白（type I collagen，COL-I）的表达。结果 与正常组比，模型组肺指数和TNF-α、TGF-β1、HYP表达均升高（P<0.05或P<0.01），肺组织炎症和肺纤维化程度较严重、α-SMA与COL-I的表达均升高。ML-HB中剂量组较模型组肺指数和TNF-α、TGF-β1、HYP表达均降低（P<0.05或P<0.01），肺组织炎症和肺纤维化程度减轻、α-SMA与COL-I的表达均降低。结论 ML-HB可降低TNF-α、TGF-β1、HYP、α-SMA和COL-I的表达，延缓肺组织病变、干预纤维化的进一步发展。

Abstract:

OBJECTIVE To investigate the protective effect of anti-pulmonary fibrosis active extract from Periplaneta Americana(ML-HB) on bleomycin-induced pulmonary fibrosis in rats. METHODS Male SD rats were randomly divided into normal group, model group, positive group(dexamethasone, 3 mg·kg^-1, qd) and ML-HB high, middle and low dosages groups (120, 60, 30 mg·kg^-1, qd). The pulmonary fibrosis model was established through tracheotomy with bleomycin(5 mg·kg^-1). The rats were intervened by treating with intraperitoneal administration after modeling 24 h. The rats were sacrificed on the 30th day after administration, lung index was determined, tumor necrosis factor-α(TNF-α) and transforming growth factor-β1(TGF-β1) in lung homogenate were detected by ELISA, hydroxyproline(HYP) was detected by UV, the inflammatory lesions of lung tissue was observed by HE staining, the degree of pulmonary fibrosis was determined by Masson staining, and α-smooth muscle actin(α-SMA) and type I collagen(COL-I) were observed by immunohistochemical staining. RESULTS Compared with the normal group, the model group showed higher lung index, and higher expression of TNF-α, TGF-β1 and HYP(P<0.05 or P<0.01), lung tissue inflammation and pulmonary fibrosis were serious, α-SMA and COL-I expression were elevated. Compared with the model group, the ML-HB middle dosage group showed lower lung index, and lower expression of TNF-α, TGF-β1, and HYP(P<0.05 or P<0.01), lung tissue inflammation and pulmonary fibrosis were alleviated, α-SMA and COL-I expression were decreased. CONCLUSION ML-HB can reduce the expression of TNF-α, TGF-β1, HYP, α-SMA and COL-I, and delay lung tissue lesions, interfere with the further development of fibrosis.