HPLC同时测定肺宁制剂中4种成分的含量

Simultaneous Determination of Four Constituents in Feining Preparation by HPLC

关键词:  肺宁颗粒  肺宁胶囊  肺宁片  新绿原酸  绿原酸  隐绿原酸  金丝桃苷  高效液相色谱法

Key words:  Feining granules  Feining capsules  Feining tablets  neochlorogenic acid  chlorogenic acid  cryptochlorogenic acid  hyperin  HPLC

摘要:

目的 建立HPLC同时测定肺宁颗粒、肺宁胶囊和肺宁片中新绿原酸、绿原酸、隐绿原酸和金丝桃苷4种成分的含量。方法 采用Waters XBridge^TMC₁₈色谱柱（4.6 mm×250 mm，5 μm），流动相为乙腈-0.4%磷酸水溶液，梯度洗脱，流速1.0 mL·min^-1；检测波长327，353 nm；柱温30℃。结果 4种成分在各自范围内线性关系良好（r>0.9990），平均加样回收率96.1%~104.7%，RSD为0.4%~1.6%。结论 该方法准确、可靠，重复性好，可用于肺宁制剂的质量控制。

Abstract:

OBJECTIVE To establish an HPLC method for the simultaneous determination of neochlorogenic acid, chlorogenic acid, cryptochlorogenic acid and hyperin in Feining granules, Feining capsules and Feining tablets. METHODS Preparation was performed on a 30℃ Waters XBridge^TM C₁₈ column(4.6 mm×250 mm, 5 μm), with the mobile phase comprising of acetonitrile-0.4% phosphoric acid flowing at 1.0 mL·min^-1 in a gradient elution manner, and the detection wavelengths were set at 327, 353 nm. RESULTS Four constituents showed good linear relationships with their own ranges(r>0.999 0), while the average recoveries were 96.1%-104.7% with the RSDs of 0.4%-1.6%. CONCLUSION This accurate, reliable and reproducible method can be used for the quality control of Feining preparation.