HPLC同时测定板蓝根颗粒中2种关键抗病毒成分的含量

Simultaneous Determination of two Antiviral Components in Banlangen Granules by HPLC

关键词:  板蓝根颗粒  (R,S)-告衣春  直铁线莲宁B  高效液相色谱法

Key words:  Banlangen granules  (R,S)-goitrin  clemastanin B  HPLC

摘要:

目的 建立HPLC同时测定板蓝根颗粒中（R，S）-告衣春和直铁线莲宁B含量的方法。方法 采用Agilent ZORBAX SB-C₁₈色谱柱（250 mm×4.6 mm，5μm），流动相为甲醇-水，梯度洗脱，流速为0.8 mL·min^-1，进样量为10μL，柱温为30℃，检测波长230 nm。结果 （R，S）-告衣春、直铁线莲宁B在2.39~47.80μg·mL^-1（r=1.000 0）和3.18~63.60μg·mL^-1（r=0.999 9）内线性关系良好，平均加样回收率分别为100.90%（RSD=1.56%）和99.48%（RSD=1.08%）。结论 本方法操作简便、准确稳定、专属性强、重复性好，可为提高板蓝根颗粒质量标准提供依据。

Abstract:

OBJECTIVE To establish a HPLC method for the simultaneous content determination of (R,S)-goitrin and clemastanin B in banlangen granules. METHODS The analysis methanol was performed on a Agilent ZORBAX SB-C₁₈ column(250 mm×4.6 mm, 5 µm), the mobile phase was methanol-water flowing of gradient elution, the flow rate was 0.8 mL·min^-1, the injection volume was 10 µL, the column temperature was 30℃, and the detection wavelength was 230 nm. RESULTS (R,S)-goitrin and clemastanin B showed good linear relationships within their own ranges of 2.39-47.80 µg·mL^-1 (r=1.000 0) and 3.18-63.60 µg·mL^-1(r=0.999 9). The average recoveries were 100.90%(RSD=1.56%) and 99.48%(RSD=1.08%). CONCLUSION This method is simple, accurate, specific and reproducible, can provide a basis for raising banlangen granules quality standards.