玫瑰红芪当归水煎剂对围绝经期大鼠IL-2含量及Sparc表达的影响

Effect of Rose, Astragalus and Angelica Decoction on IL-2 Contents and Sparc Expression in Perimenopausal Rats

关键词:  玫瑰红芪当归水煎剂  围绝经期  内分泌激素  免疫功能  富含半胱氨酸的酸性分泌蛋白

Key words:  rose,astragalus and angelica decoction  perimenopause  endocrine hormones  immunologic function  secreted protein acidic and rich in cysteine

摘要:

目的 观察玫瑰红芪当归水煎剂对围绝经期大鼠IL-2含量及Sparc表达变化的影响。方法 通过手术切除Wistar ♀大鼠双侧卵巢复制围绝经期综合征模型。造模后第7天用ELISA法检测血清雌二醇（estradiol，E2）含量确定造模是否成功，于造模成功后第2周将切除卵巢的大鼠随机分为模型组，玫瑰红芪当归水煎剂低、中、高剂量组（1.4，2.8，5.6 g·kg^-1）和戊酸雌二醇片组（0.09 mg·kg^-1），每组10只。另设10只Wistar ♀大鼠作为正常组。末次给药24 h后，腹主动脉采血处死大鼠后，称取各组大鼠体质量，并计算其子宫指数及胸脾指数，HE染色法观察各组大鼠子宫组织形态学变化，ELISA法测定各组大鼠血清E2和白细胞介素-2（interleukin-2，IL-2）的含量，实时荧光定量PCR法检测各组大鼠富含半胱氨酸的酸性分泌蛋白（secreted protein acidic and rich in cysteine，Sparc）的基因表达，免疫组化法观察各组大鼠雌激素受体α（estradiol receptor α，ERα）的蛋白表达。结果 与模型组相比，玫瑰红芪当归水煎剂中、高剂量组和戊酸雌二醇片组能明显降低大鼠的体质量，明显升高大鼠的子宫指数、胸腺指数、脾脏指数、Sparc的基因表达和ERα的蛋白表达（P<0.05）；玫瑰红芪当归水煎剂各治疗组和戊酸雌二醇片组能明显升高模型组大鼠E2含量和IL-2的含量（P<0.05）。HE染色发现，玫瑰红芪当归水煎剂高剂量组和戊酸雌二醇片组子宫黏膜层出现腺体，单层柱状上皮细胞排列整齐。结论 玫瑰红芪当归水煎剂治疗围绝经期综合征与调节机体内分泌-免疫系统有关，为临床应用和新药开发提供实验依据。

Abstract:

OBJECTIVE To observe the effect of rose, astragalus and angelica decoction on the changes of IL-2 and secreted protein acidic and rich in cysteine(Sprac) expression during perimenopause period in rats. METHODS Peri-menopausal syndrome models were established by surgical removal of the bilateral ovaries of female Wistar rats. After successful modeling by detecting the content of estradiol(E2) by ELISA on the 7th postoperative day, the rats were randomly divided into model group, rose, astragalus and angelica decoction low, medium and high dose groups(1.4, 2.8, 5.6 g·kg^-1) and estradiol valerate tablet group(0.09 mg·kg^-1) in the second week after successful modeling, each for 10 rats. Another 10 female Wistar rats were picked as the normal group. 24 h after the last administration, abdominal aortic blood was took to measure the indexes of the uterus, thymus and spleen was checked by weighing method, HE staining to observe uterus tissue morphology, the concentration of E2 and IL-2 in serum was measured by ELISA, Sparc gene expression of uterus tissue was checked by real-time fluorescence quantitative PCR, estradiol receptor α(ERα) protein expression of uterus tissue was determined by immunohistochemistry. RESULTS Compared with the model group, the weight of rats in rose, astragalus and angelica decoction middle, high dose group and estradiol valerate tablet group was significantly lower, the index of uterus, thymus and spleen were significantly higher, the gene expression of Sparc was raised and the protein of ERα were also significantly higher(P<0.05); serum E2 and IL-2 in rose, astragalus and angelica decoction treatment group and estradiol valerate tablet group were significantly higher(P<0.05). The glands appeared and monolayer columnar epithelial cells arranged neatly in high dose group of astragalus and angelica decoction treatment group and estradiol valerate tablet group by HE staining. CONCLUSION Rose, astragalus and angelica decoction can regulate beneficially the unbalanced reproductive endocrine-immune net-work. It can provide experimental basis for clinical application and new perimenopause drugs development.