茵陈五苓散对肝硬化腹水大鼠模型的影响

Effect of Yinchen Wuling Powder on Cirrhotic Rats Model with Ascites

关键词:  茵陈五苓散  肝硬化腹水  AQP2  NO  ET-1

Key words:  Yinchen Wuling powder  cirrhotic with ascites  AQP2  NO  ET-1

摘要:

目的 研究茵陈五苓散对肝硬化腹水大鼠血清AQP2表达和NO、NT-1水平的影响。方法 采用苯巴比妥灌胃联合四氯化碳腹腔注射复制肝硬化腹水模型。将成模后大鼠随机分为模型组、螺内酯片组（0.066 g·kg^-1）和茵陈五苓散低、中、高剂量组（生药4.3，8.6，17.2 g·kg^-1），每组10只，并选取10只正常大鼠灌服生理盐水作为空白组。硝酸还原酶法测定大鼠血清NO、ET-1的水平。RT-PCR测定大鼠肝组织AQP2 mRNA水平，Western blotting检测大鼠肝组织AQP2蛋白含量。结果 与空白组大鼠比较，模型组大鼠血浆NO和ET-1水平显著升高（P<0.05）。与模型组大鼠比较，茵陈五苓散各剂量组和螺内酯片组大鼠血清NO、ET-1水平显著降低（P<0.05）。与空白组大鼠比较，模型组大鼠肝脏AQP2 mRNA和蛋白含量显著下降（P<0.05）。与模型组大鼠比较，茵陈五苓散各剂量组和螺内酯片组大鼠肝脏AQP2 mRNA和蛋白含量显著增高（P<0.05）。结论 茵陈五苓散治疗肝硬化腹水的机制与降低血清NO、ET-1水平及调节肝脏AQP-2基因表达有关。

Abstract:

OBJECTIVE To study the effect of Yinchen Wuling powder on AQP2 expression and the plasma levels of NO as well as ET-1 in cirrhotic rats with ascites. METHODS The ascites model of cirrhosis was established by intraperitoneal injection of phenobarbital and carbon tetrachloride. The rats were randomly divided into model group, colchicine group, and low, middle and high-dose of Yinchen Wuling powder groups(crude drug 4.3, 8.6, 17.2 g·kg^-1). Ten rats in each group, and ten normal rats were given normal saline as blank group. Serum levels of NO and ET-1 were measured by nitrate reductase method. The expression of mRNA and protein of AQP2 was measured by RT-PCR and Western blotting method respectively. RESULTS Compared with the blank group, the plasma levels of NO and ET-1 in the model group increased significantly(P<0.05). Compared with the model group, the plasma levels of NO and ET-1 in Yinchen Wuling powder treat groups and colchicine group decreased significantly(P<0.05). Compared with the blank group, the expression of mRNA and protein of AQP2 in the liver of the model group were significantly decreased(P<0.05). Compared with the model group, the expression of mRNA and protein of AQP2 in liver of rats in Yinchen Wuling powder treat groups and colchicine group were significantly increased(P<0.05). CONCLUSION The mechanism of Yinchen Wuling powder in treating cirrhotic rats with ascites may be related to reduction of plasma levels of NO as well as ET-1 and the regulation of AQP2 gene expression.