蒙药巴特日七味丸不同提取部位体外抗菌活性筛选和急性毒性研究

Study on the in Vitro Antimicrobial Activity Screening for Different Extracts of Bateri Qiwei Pills and Acute Toxicity

关键词:  蒙药  巴特日七味丸  体外抗菌  作用部位  急性毒性

Key words:  Mongolian medicine  Bateri Qiwei pills  antibiotic in vitro  site of action  acute toxicity

摘要:

目的 考察蒙成药巴特日七味丸不同提取部位对临床常见7种致病菌的体外抗菌活性，并对筛选出的有效活性部位进行急性毒性评价。方法 采用微孔板TTC法和微孔板比浊法测定吸光度（OD）值和最低抑菌浓度（minimum inhibitory concentration，MIC），观察巴特日七味丸不同提取部位的抗菌作用，并初步确定其有效活性部位；以巴特日七味丸水提物为对照，采用小鼠半数致死量（half lethal dose，LD₅₀）和最大耐受量试验对巴特日七味丸有效活性部位进行急性毒性评价。结果 巴特日七味丸6种提取部位，包括石油醚、氯仿、乙酸乙酯、正丁醇、乙酸乙酯-正丁醇和水残余等部位均具有一定的抗菌活性，其中乙酸乙酯-正丁醇部位具有较强的抗菌活性，并对金黄色葡萄球菌和肠炎沙门氏菌的抑菌作用较强，MIC分别为0.04，0.08 mg·mL^-1。巴特日七味丸水提物和有效活性部位均没有出现LD₅₀值；最大耐受量相当于临床成人（体质量70 kg）安全用药剂量的120倍左右，均未出现急性毒性现象。结论 巴特日七味丸不同提取部位均具有抗菌活性，其乙酸乙酯-正丁醇部位活性较强，并且急性毒性较低，其作用机制有待于进一步研究。

Abstract:

OBJECTIVE To screening the antibacterial activity of different extracts of Mongolian medicine Bateri Qiwei pills against 7 common clinical pathogenic bacteria in vitro, and assessing the acute toxicity for the selected effective parts. METHODS The antimicrobial effect of the different extracts of the Bateri Qiwei pills was investigated according to the absorbance value and the minimum inhibitory concentration(MIC) measured by the methods of TTC and turbidimetric on micropore plate, and the effective parts of Bateri Qiwei pills were confirmed preliminarily by the above tests. Using the water extract of Bateri Qiwei pills as contrast, the acute toxicity of effective parts of Bateri Qiwei pills was evaluated by the experiment of half lethal dose(LD₅₀) and maximum tolerance in mice. RESULTS The six extracted parts of Bateri Qiwei pills, including petroleum ether, chloroform, ethyl acetate, n-butanol, ethyl acetate-n-butanol and water residue, had certain antibacterial activity. Among them, the parts of ethyl acetate-n-butanol had strong antimicrobial activity, especially to Staphylococcus aureus and Salmonella enteritidis, the value of MIC were 0.04 mg·mL^-1 and 0.08 mg·mL^-1, respectively. There were no LD₅₀ value for the water extract and the effective part of Bateri Qiwei pills appeared in the tests. And the maximum tolerance dosage in mice was 120 times of the clinical safe dosage in human(70 kg), and acute toxicity was not found. CONCLUSION The different extracts of Bateri Qiwei pills all have antibacterial activity, and the parts of ethyl acetate-n-butanol of Bateri Qiwei pills have strong antibacterial activity. Acute toxicity evaluation of the selected effective sites is relatively low, its mechanism of action needs to be further studied.