新疆野生樱桃李中多糖提取物提取工艺及其抗氧化活性研究

Study on Extraction Technology and Antioxidant Activity of Polysaccharide Extract from Wild Prunus Cerasifera

关键词:  樱桃李多糖  超声波法  响应面  红外光谱  抗氧化

Key words:  Prunus cerasifera polysaccharides  ultrasonic method  response surface  IR  antioxidant

摘要:

目的 采用Box-Behnken法优化樱桃李中多糖提取物的提取工艺，并考察其抗氧化活性。方法 分别考察提取时间、提取温度及料液比对多糖提取物得率的影响，采用三因素三水平响应面法进行多糖提取物提取工艺的优化，利用红外光谱分析其结构。结果 樱桃李中多糖提取物最佳提取工艺条件为料液比1∶30，提取温度为73℃，超声时间为61 min。在此条件下，樱桃李中多糖提取物得率为7.20%，樱桃李果肉中多糖含量为1.41%。红外光谱分析显示，提取物中有糖醛酸和α-D-吡喃糖。在一定的浓度范围内，樱桃李中多糖具有DPPH自由基和羟自由基清除能力，即具有一定抗氧化活性。结论 超声提取操作简便易行，樱桃李果肉中含有较丰富的多糖，提取的樱桃李粗多糖具有较好的抗氧化活性。

Abstract:

OBJECTIVE To optimize the extraction technology of polysaccharides extract from Prunus cerasifera by Box-Behnken designing, and to investigate its antioxidant activity. METHODS The effects of extraction time, extraction temperature and liquid-to-material ratio on the yield of polysaccharide extract were established using a 3-factor, 3-lever Box-Behnken design and the structure of polysaccharides was analyzed by IR. RESULTS The optimal extraction condition was obtained as extraction time 61 min, extraction temperature 70℃, liquid-to-material ratio 1:30, ultrasonic time 61 min. Under such condition, the yield of polysaccharides from Prunus cerasifera was 7.20%, and content was 1.41%. Infrared spectroscopy analysis, extracts were glycolic acid and α-D-pyranose. In a certain concentration range, the polysaccharides of Prunus cerasifera had DPPH free radical and hydroxyl radical scavenging ability, showed certain antioxidant activity. CONCLUSION Ultrasonic extraction is simple and easy, and there are abundant polysaccharides in the flesh of Prunus cerasifera plum. The extracted crude polysaccharides from Prunus cerasifera plum have good antioxidant activity.