畲药地稔及其易混淆品的鉴别

Identification of She Medicine of Melastoma Dodecandrum Lour. and Its Adulterants

关键词:  地稔  混淆品  显微鉴别  高效液相色谱法  指纹图谱

Key words:  Melastoma dodecandrum Lour.  adulterants  microscopic identification  HPLC  fingerprint

摘要:

目的 为防止因误用混淆品而降低临床疗效，建立畲药地稔及其易混淆品的鉴别方法。方法 采用显微鉴别法进行地稔及其易混淆品秀丽野海棠、中华野海棠、方枝野海棠药材组织结构的鉴别；基于HPLC指纹图谱，建立地稔及其上述混淆品的液相色谱鉴别方法。结果 地稔根横切面皮层石细胞、栓内层簇晶均较多，为其与易混淆品的主要显微区别；基于HPLC指纹图谱，地稔及其易混淆品的区别主要有2点：一是相对于没食子酸峰相对保留时间约为1.1的色谱峰为不同的2种化学物质；二是上述化学物质的色谱峰与没食子酸峰的峰面积比值相差较大，地稔中该比值均>0.90，平均值为1.35，而其易混淆品中该比值均<0.30。结论 建立的显微和液相鉴别方法能快速地将地稔及其易混淆品进行区别，并且操作简单，易于推广应用，具有广阔的应用前景。

Abstract:

OBJECTIVE To prevent the clinical effect from being reduced due to the misuse of adulterants, the method was established to identify She medicine of Melastoma dodecandrum Lour. and its adulterants. METHODS M. dodecandrum Lour. and its adulterants: Bredia amoena Diels., Bredia sinensis (Diels) H. L. Li, Bredia quadrangularis Cogn. were conducted by microscopic identification and a liquid phase identification method was established based on the fingerprint of HPLC. RESULTS The main microscopic differences between M.dodeccandrum Lour. and its adulterants were summarized as follows:in root transverse section of the authentic materals contained the relatively large number of sclereid in phellem and clusters of calcium oxalate in phelloderm. There were two main differences to HPLC based on HPLC fingerprint:One was the chemicals with the relative retention time of gallic acid peaks which were about 1.1 were different in authentic and confusing products. Second was the ratio of chromatographic peak area to gallic acid peak area of the above chemical substances was quite different:The ratio in the authentic materials was > 0.90, the average value was 1.35, and the ratio in the easily confused products was < 0.30. CONCLUSION The methods established above distinguish the M. dodeccandrum Lour. and its adulterants quickly, simple and convenient, and easy to generalize application, has a wide application prospects.