都梁丸的血清药物化学研究

Study on Serum Pharmacochemistry of Duliang Wan

关键词:  都梁丸  血清药物化学  高效液相色谱法  入血成分

Key words:  Duliang Wan  serum pharmacochemistry  HPLC  absorbed compounds in the blood

摘要:

目的 对都梁丸的入血成分进行分析研究，探讨都梁丸的药效物质基础。方法 采用偏头痛模型小鼠，灌胃给药制得含药血清和空白血清，通过比较都梁丸、酒炖白芷和川芎体外供试品及含药血清样品和空白血清样品的HPLC色谱图，根据保留时间及对照品，明确都梁丸给药后血中移行成分。结果 都梁丸给药后，在血清中共有22个移行成分，其中18个成分为原形入血成分，4个成分为原型成分的代谢产物，经对照品确认了12种原型成分。结论 这22个血中移行成分可能是都梁丸在体内直接作用的药效物质，为深入研究其作用机制、质量控制标准及剂型改革提供了科学依据。

Abstract:

OBJECTIVE To study the blood compounds in DulingWan and to further explore its pharmacodynamic basis. METHODS The drug-contaning serum and blank serum were prepared by orally administration of migraine mice. Rapid identification of compounds absorbed into blood was carried out by HPLC, according to retention time and reference substances comparison, then preliminary determination of constituents absorbed into blood of mice after oral administration of Duliang Wan, Stewed Angelica dahurica and Ligusticum chuanxiong was investigated. RESULTS After the administration of Duliang Wan, totally 22 compounds absorbed into blood were detected in serum, 18 of them were prototype compounds and 4 components were metabolites of the prototype components. And 12 prototype components were confirmed by reference substance. CONCLUSION These 22 compounds absorbed into blood may be substances that act directly in vivo of Duliang Wan, and it is helpful to clarify mechanism of this herb, quality control standard and dosage form reform.