京尼平苷体内外微透析回收率的研究

Microdialysis Recovery Study of Geniposide in Vitro and in Vivo

关键词:  京尼平苷  微透析  探针回收率  体外  体内  皮肤

Key words:  geniposide  microdialysis  probe recovery  in vitro  in vivo  skin

摘要:

目的 探究京尼平苷在自制线性微透析探针上的体内外回收率与灌流速度、药物浓度、透析膜长之间的关系。方法 采用HPLC测定微透析样品浓度，考察不同灌流速度、不同药物浓度和不同透析膜长对体外正向和体外反向回收率、体内反向回收率的影响。结果 自制探针性质稳定；探针回收率与京尼平苷的药物浓度无关，与灌流速度成反比，随透析膜长增加而增加；体外的正向与反向探针回收率有显著性差异（P<0.01），而体内反向回收率低于体外反向回收率，与体外正向回收率无显著性差异；新探针体内回收率大于使用过1次的探针体内回收率（P<0.05）。结论 在京尼平苷的皮肤药动学研究中，宜采用体内反向回收率或体外正向回收率作为药物的探针回收率来校正。

Abstract:

OBJECTIVE To investigate the relationship between the recovery of geniposide on self-made linear microdialysis probes and flow rates, drug concentrations, and dialysis membrane lengths. METHODS The concentrations of microdialysis samples were determined by HPLC. The effects of different perfusion speed, different drug concentration and different dialysis membrane length on the in vitro and in vitro forward, reverse recovery and in vivo reverse recovery were investigated. RESULTS The self-made probe was stable. The microdialysis recovery of geniposide was independent of geniposide concentrations, inversely proportional to the flow rates and increased as the length of the dialysis membrane lengths increased. The recovery of positive dialysis and retrodialysis in vitro was significant different(P<0.01). The recovery of in vivo retrodialysis was lower than that of in vitro retrodialysis but was similar to that of in vitro positive dialysis. The recovery of new probe was higer than that used one time in vivo(P<0.05). CONCLUSION It is advisable to use the recovery of in vivo retrodialysis or in vitro positive dialysis method for geniposide skin pharmacokinetic study.