| 引用本文: | 周君卓,董武军,刘玉玲.络合沉淀-紫外分光光度法测定黄芩素脂肪乳中甲氧基苯胺值[J].中国现代应用药学,2019,36(24):3019-3022. |
| ZHOU Junzhuo,DONG Wujun,LIU Yuling.Determination of the Anisidine Value in Baicalein Lipid Emulsions by Complex Precipitation-UV Spectrophotometry Method[J].Chin J Mod Appl Pharm(中国现代应用药学),2019,36(24):3019-3022. |
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| 摘要: |
| 目的 建立一种专属性好，适用于主药有干扰的脂肪乳中甲氧基苯胺值的测定方法。方法 以醋酸铅为络合沉淀剂，采用络合沉淀法排除主药黄芩素对甲氧基苯胺值测定的影响，并对改进后的方法进行验证分析。结果 醋酸铅对甲氧基苯胺值测定结果无影响，加入量为200 μL （0.4 g·mL-1）时，可排除药物干扰，干扰试验平均回收率为99.39%，RSD为1.73%（n=3），重复性RSD均<2.0%（n=5）。结论 该方法专属性好，测定结果准确可靠，能有效排除药物对脂肪乳甲氧基苯胺值测定的影响，为载药脂肪乳中甲氧基苯胺值控制提供了新的思路和方法。 |
| 关键词: 甲氧基苯胺值 络合沉淀法 黄芩素 脂肪乳 |
| DOI：10.13748/j.cnki.issn1007-7693.2019.24.003 |
| 分类号:R284.1 |
| 基金项目:“重大新药创制”国家科技重大专项（2018ZX09721-003） |
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| Determination of the Anisidine Value in Baicalein Lipid Emulsions by Complex Precipitation-UV Spectrophotometry Method |
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ZHOU Junzhuo, DONG Wujun, LIU Yuling
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Institute of Materia Medica, Chinese Academy of Medical Sciences&Peking Union Medical College, Beijing Key Laboratory of Drug Delivery Technology and Novel Formulations, Beijing 100050, China
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| Abstract: |
| OBJECTIVE To establish an accurate and reliable method for determining the anisidine value in baicalein lipid emulsions. METHODS Used lead acetate as a complexing precipitator, the complex precipitation method was used to exclude the effect of the baicalein on the determination of anisidine value, and the improved method was verified and analyzed. RESULTS The content of precipitator was 200 μL(0.4 g·mL-1), and the method was specific. The result of interference test was 99.39%(RSD=1.73%, n=3). The repeatability RSD was <2.0%(n=5). CONCLUSION The method is specific, accurate and reliable, and can effectively eliminate the influence of drug on the determination of the anisidine value in lipid emulsions, which provides a new idea and approach for the control of the anisidine value in lipid emulsions. |
| Key words: anisidine value complex precipitation baicalein lipid emulsions |
