| 引用本文: | 刘永琴,罗忠圣,徐昌艳,钱志瑶,杨宏,覃容贵.基于酪氨酸酶抑制作用的白术水提工艺研究[J].中国现代应用药学,2020,37(10):1196-1202. |
| LIU Yongqin,LUO Zhongsheng,XU Changyan,QIAN Zhiyao,YANG Hong,QIN Ronggui.Study on Water Extraction Technology of Atractylodis Macrocephalae Rhizoma Based on Inhibitory Effect of Tyrosinase[J].Chin J Mod Appl Pharm(中国现代应用药学),2020,37(10):1196-1202. |
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| 基于酪氨酸酶抑制作用的白术水提工艺研究 |
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刘永琴1, 罗忠圣2,3, 徐昌艳1, 钱志瑶4, 杨宏1, 覃容贵1
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1.贵州医科大学, 药学院, 贵阳 550025;2.贵州省中国科学院天然产物化学重点实验室(贵州医科大学天然产物化学重点实验室), 贵阳 550014;3.省部共建药用植物功效与利用国家重点实验室, 贵州医科大学, 贵阳 550014;4.贵州医科大学, 公共卫生学院, 贵阳 550025
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| 摘要: |
| 目的 优化白术的提取工艺。方法 在单因素试验基础上，利用响应面法以料液比、提取次数、提取时间为因素，提取物的产率、提取物对酪氨酸酶的抑制率为响应值，确定白术的最佳水提工艺，研究其美白效果。结果 白术的最佳提取工艺条件为料液比1：14，提取次数3次，提取时间2.5 h，在此条件下白术提取物的产率可达到64.4%；提取物对酪氨酸酶的抑制率可达到65.16%。结论 白术提取物的产率、提取物对酪氨酸酶的抑制率的实测值和预测值基本一致，具有较好的美白效果。 |
| 关键词: 响应面法 白术 酪氨酸酶 提取工艺 |
| DOI：10.13748/j.cnki.issn1007-7693.2020.10.008 |
| 分类号:R284.2 |
| 基金项目:贵州省2019年大学生创新创业训练计划项目（20195200925） |
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| Study on Water Extraction Technology of Atractylodis Macrocephalae Rhizoma Based on Inhibitory Effect of Tyrosinase |
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LIU Yongqin1, LUO Zhongsheng2,3, XU Changyan1, QIAN Zhiyao4, YANG Hong1, QIN Ronggui1
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1.Guizhou Medical University, Pharmacy School, Guiyang 550025, China;2.The Key Laboratory of Chemistry for Natural Products of Guizhou Province and Chinese Academy of Sciences(The Key Laboratory of Natural Product Chemistry, Guizhou Medical University), Guiyang 550014, China;3.State Key Laboratory of Functions and Applications of Medicinal Plants, Guizhou Medical University, Guiyang 550014, China;4.Guizhou Medical University, School of Public Health, Guiyang 550025, China
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| Abstract: |
| OBJECTIVE To optimize the extraction technology of Atractylodis Macrocephalae Rhizoma. METHODS Using water/solid ratio, extraction times, extraction duration as factors, the yield and the inhibitory activity against tyrosinase of the extracts as response value, reponse surface method was used to obtain the optimal extraction technology of Atractylodis Macrocephalae Rhizoma based on single factor test and its whitening effect was tested. RESULTS The result showed that the optimal conditions were:the water/solid ratio of 1:14, the extraction time of 3, extraction duration of 2.5 h. Under the optimal condition, the yield of Atractylodis macrocephalae rhizoma extract was 64.4% and the inhibition rate of extract on tyrosinase was 65.16%. CONCLUSION The yield of Atractylodis Macrocephalae Rhizoma extract and the inhibition rate of extract on tyrosinase are basically similar with the predicted values. It has good whitening effect. |
| Key words: response surface methodology Atractylodis Macrocephalae Rhizoma tyrosinase extraction technology |
