藏药青叶胆对实验性慢性肝损伤小鼠T淋巴细胞亚群的影响

Effect of Tibetan Medicine Swertia Mileensis T. M. Host W. L. Shih on T Lymphocyte Subsets in Mice with Experimental Chronic Liver Injury

关键词:  藏药  青叶胆  慢性肝损伤  T淋巴细胞亚群  免疫调节

Key words:  Tibetan medicine  Swertia mileensis T. M. Host W. L. Shih  chronic liver injury  T lymphocyte subsets  immunomodulation

摘要:

目的 观察青叶胆对小鼠慢性肝损伤的保护作用及其对小鼠脾脏T淋巴细胞亚群的调节作用。方法 采用腹腔注射四氯化碳（CCl₄）造成小鼠化学性肝损伤模型，检测小鼠血清天冬氨酸转氨酶（aspartate aminotransferase，AST）、丙氨酸转氨酶（alanine aminotransferase，ALT）、总蛋白（total protein，TP）及总胆红素（total bilirubin，TBI）；HE染色，观察肝组织形态；流式细胞术检测CD3⁺、CD4⁺及CD8⁺阳性细胞数的变化情况，计算CD4⁺/CD8⁺比例，并与模型组和对照组比较。结果 青叶胆对CCl₄造成的小鼠慢性肝脏损伤具有保护作用，与模型组相比，能明显降低小鼠血清ALT、AST含量（P<0.01），升高TP含量（P<0.01）；与模型组相比，青叶胆低剂量组对CD3⁺细胞具有下调作用（P<0.05），对CD8⁺细胞的上调作用和对CD4⁺/CD8⁺比值的下调作用具有显著性差异（P<0.01）；高剂量组对CD8⁺细胞的上调作用和对CD4⁺/CD8⁺比值的下调作用具有统计学意义（P<0.05）。结论 青叶胆对CCl₄诱导的小鼠慢性肝损伤具有保护作用，其机制可能是通过调节T淋巴细胞亚群，提高免疫功能。

Abstract:

OBJECTIVE To observe the protective effect of Swertia mileensis T. M. Host W. L. Shih on chronic liver injury in mice and its regulation on spleen T lymphocyte subsets in mice. METHODS A model of chemical liver injury in mice was induced by intraperitoneal injection of carbon tetrachloride(CCl₄). Serum aspartate aminotransferase(AST), alanine aminotransferase(ALT), total protein(TP) and total bilirubin(TBI) were detected in mice. HE staining was used to observe liver tissue morphology; flow cytometry was used to detect changes in the number of CD3⁺, CD4⁺ and CD8⁺ positive cells; the proportion of CD4⁺/CD8⁺ ratio were calculated, and they were compare with the model group and the control group. RESULTS S. mileensis had protective effects on chronic liver injury induced by CCl₄ in mice, which could significantly decrease serum ALT and AST levels(P<0.01) and increase TP content compared with model group(P<0.01). Compared with model group, the low dose group of S. mileensis had a down-regulating effect on CD3⁺ cells(P<0.05), and there was a significant difference in the up-regulating effect on CD8⁺ cells and the down-regulating effect on the ratio of CD4⁺/CD8⁺(P<0.01); the high dose group of S. mileensis had a statistically significant effect on the up-regulation of CD8⁺ cells and the down-regulation of CD4⁺/CD8⁺ ratio(P<0.05). CONCLUSION S. mileensis has protective effect on CCl₄-induced chronic liver injury in mice, and its mechanism may be through the regulation of T lymphocyte subsets, affecting immune function.