| 引用本文: | 张昆艳,洪挺,王栋,简勇学,赵凯,黄建丽,杨毅生.HPLC同时测定细梗香草中2个专属性黄酮苷的含量[J].中国现代应用药学,2020,37(13):1570-1573. |
| ZHANG Kunyan,HONG Ting,WANG Dong,JIAN Yongxue,ZHAO Kai,HUANG Jianli,YANG Yisheng.Simultaneous Determination of Two Specific Flavonoid Glycosides in Lysimachia Capillipes Hemsl. by HPLC[J].Chin J Mod Appl Pharm(中国现代应用药学),2020,37(13):1570-1573. |
|
| |
|
|
| 本文已被：浏览 1805次 下载 895次 |
码上扫一扫！ |
|
|
| HPLC同时测定细梗香草中2个专属性黄酮苷的含量 |
|
张昆艳1,2, 洪挺1, 王栋3, 简勇学4, 赵凯4, 黄建丽4, 杨毅生1
|
|
1.江西省药品检验检测研究院, 江西省药品与医疗器械质量工程技术研究中心, 南昌 330029;2.江西中医药大学, 南昌 330004;3.江西省食品检验检测研究院, 江西国家果蔬产品及加工食品质量监督检验中心, 南昌 330001;4.江西欧氏药业有限责任公司, 江西 新余 338004
|
|
| 摘要: |
| 目的 建立HPLC同时测定细梗香草中Quercetin-3-O-（2''，6''-di-O-α-rhamnopyranosyl）-β-galactopyranoside和Kaempferol-3-O-[2-glucopyranosyl （1→3）rhamnopyranosyl-6-rhamnopyranosyl]-β-D-galactopyranoside含量的方法。方法 采用SHIMADZU Shim-pack VP-ODS C18色谱柱（250 mm×4.6 mm，5 μm），乙腈-0.3%磷酸溶液为流动相，线性梯度洗脱，流速为1 mL·min-1；柱温为30℃，检测波长为350 nm。结果 Quercetin-3-O-（2''，6''-di-O-α-rhamnopyranosyl）-β-galactopyranoside和Kaempferol-3-O-[2-glucopyranosyl（1→3）rhamnopyranosyl-6-rhamnopyranosyl]-β-D-galactopyranoside分别在0.041 46~2.072 8 μg·mL-1（r=0.999 9），0.088 95~4.447 5 μg·mL-1（r=0.999 9）内呈良好的线性关系，平均加样回收率（n=6）分别为98.19%，98.85%；RSD分别为1.94%，1.81%。结论 建立的方法操作简单、专属性强，可用于同时测定细梗香草中2个专属黄酮苷类成分含量。 |
| 关键词: 细梗香草 高效液相色谱法 黄酮苷 含量测定 |
| DOI：10.13748/j.cnki.issn1007-7693.2020.13.006 |
| 分类号:R917.101 |
| 基金项目:江西省重点研发计划项目（20161BBG70236）；江西省食品药品监督管理局科技计划项目（2015YP08）；国家药典委员会2015年国家药品标准提高项目（163） |
|
| Simultaneous Determination of Two Specific Flavonoid Glycosides in Lysimachia Capillipes Hemsl. by HPLC |
|
ZHANG Kunyan1,2, HONG Ting1, WANG Dong3, JIAN Yongxue4, ZHAO Kai4, HUANG Jianli4, YANG Yisheng1
|
|
1.Jiangxi Provincial Institute for Drug Control, Jiangxi Provincial Engineering Research Center for Drug and Medical Device Quality, Nanchang 330029, China;2.Jiangxi University of Traditional Chinese Medicine, Nanchang 330004, China;3.Food Inspection and Testing Institute of Jiangxi Province, Jiangxi National Center for Quality Supervision and Inspection of Fruits, Vegetables, and Processed Food, Nanchang 330001, China;4.Jiangxi Ourshi Pharmaceutical Co., Ltd, Xinyu 338004, China
|
| Abstract: |
| OBJECTIVE To establish an HPLC method for the simultaneous determination of Quercetin-3-O-(2'', 6''-di-O- α-rhamnopyranosyl)-β-galactopyranoside and Kaempferol-3-O-[2-glucopyranosyl(1→3)rhamnopyranosyl-6-rhamnopyranosyl]- β-D-galactopyranoside in Lysimachia capillipes Hemsl. METHODS The HPLC analysis was carried on SHIMADZU Shim- pack VP-ODS C18 column(250 mm×4.6 mm, 5 μm) with mobile phase consisted of acetonitrile-0.3% phosphoric acid aqueous solution (gradient elution) at the flow rate of 1 mL·min-1. The column temperature was set at 30℃, and the detection wavelength was set at 350 nm. RESULTS Quercetin-3-O-(2'',6''-di-O-α-rhamnopyranosyl)-β-galactopyranoside and Kaempferol-3-O-[2- glucopyranosyl(1→3)rhamnopyranosyl-6-rhamnopyranosyl]-β-D-galactopyranoside were showed good linear relationships within the ranges of 0.041 46-2.072 8 μg·mL-1(r=0.999 9) and 0.088 95-4.447 5 μg·mL-1(r=0.999 9). The average recoveries (n=6) were 98.19% and 98.85%, RSDS were 1.94% and 1.81%, respectively. CONCLUSION The established method is simple, specific, and suitable for simultaneous determination of two components of specific flavonoid glycosides in Lysimachia capillipes Hemsl. |
| Key words: Lysimachia capillipes Hemsl HPLC flavonoid glycosides component determination |
|
|
|
|
