灰树花多糖体外抑菌及抗氧化活性研究

Study on Antibacterial in Vitro and Antioxidant Activity of Grifola Frondosa Polysaccharide

关键词:  灰树花  多糖  抑菌  抗氧化

Key words:  Grifola frondosa  polysaccharides  antibacterial  antioxidant activity

摘要:

目的 研究灰树花多糖的抑菌及抗氧化活性。方法 采用紫外分光光度法，以D-无水葡萄糖为对照品，测定灰树花提取物中多糖含量；采用纸片扩散法，以头孢哌酮为阳性对照，测量不同浓度灰树花多糖提取物金黄色葡萄球菌、大肠杆菌、枯草芽孢杆菌的抑菌圈直径；采用DPPH法和T-AOC试剂盒法，以清除率为指标，研究灰树花多糖的抗氧化活性。结果 灰树花提取物中多糖含量为53.49%；灰树花提取物对金黄色葡萄球菌、大肠杆菌、枯草芽孢杆菌均有一定的抑菌作用，抑菌圈直径分别为13，11，10 mm；灰树花多糖浓度为0.05~10 mg·mL^-1时，对DPPH自由基具有一定的清除能力，当浓度高于0.5 mg·mL^-1时，开始显示出总抗氧化能力。结论 灰树花多糖具有一定的抑菌和抗氧化能力。

Abstract:

OBJECTIVE To study the antibacterial effect and antioxidant activity of Grifola frondosa polysaccharide. METHODS UV spectrophotometry was used to determine the content of polysaccharide, D-anhydroglucose was used as a standard. The antibacterial effect produced by Kirby-Baue method with the bacteria of Staphylococcus aureus, Escherichia coli and Bacillus subtilis. Took the scavenging capacity as index to estimate the endogenous antioxidative activity of DPPH and T-AOC. RESULTS The total polysaccharides in Grifola frondosa extract was 53.82%. Grifola frondosa polysaccharide had a good antibacterial effect on Staphylococcus aureus, Escherichia coli and Bacillus subtilis, the diameter of antibacterial were 13, 11, 10 mm. With the concentration between 0.05~10 mg·mL^-1, Grifola frondosa polysaccharide had a good scavenging activity by DPPH, when the concentration above 0.5 mg·mL^-1, showed the total antioxidant activity. CONCLUSION The polysaccharides in Grifola frondosa has certain antibacterial effect and antioxidant activity.