对照品替代法用于莪术油中3个倍半萜类成分的含量测定研究

Studies on the Determination of Three Sesquiterpenoids in the Zedoary Turmeric Oil by Substitution of Reference Substance Method

关键词:  对照品替代法  倍半萜类成分  莪术油

Key words:  substitution of reference substance method(SRSM)  sesquiterpenoid  Zedoary Turmeric oil

摘要:

目的 建立对照品替代法（substitution of reference substance method，SRSM）测定莪术油中3种倍半萜类成分的含量。方法 采用C₁₈色谱柱（4.6 mm×250 mm，5 μm），以乙腈-水为流动相，梯度洗脱，流速为1.0 mL·min^-1，检测波长为216 nm，柱温为30℃。以牻牛儿酮为参照物，计算呋喃二烯、莪术二酮的相对校正因子和相对保留时间，采用外标法和SRSM分别测定它们在莪术油中的含量。结果 3种成分在各自范围内线性关系良好（R² ≥ 0.999 9），相对校正因子及相对保留时间稳定，SRSM测定结果与外标法测定结果无显著性差异。结论 建立的SRSM操作简单、重复性好，能够替代外标法用于莪术油中3种倍半萜类成分的含量测定。

Abstract:

OBJECTIVE To develop substitution of reference substance method(SRSM) for simultaneous determination of furanodiene, curdione and germacrone in the Zedoary Turmeric oil. METHODS The determination of furanodiene, curdione and germacrone were performed on a C₁₈ analytical column(4.6 mm×250 mm, 5 μm) with acetonitrile-water as mobile phase in a gradient elution program at a flow rate of 1.0 mL·min^-1. The column temperature was maintained at 30℃. The detection wavelength was 216 nm. Germacrone was used as the internal reference substance, RCFs and RRTs of furanodiene and curdione were calculated. The contenets of furanodiene and curdione were determined by both the external standard method(ESM) and SRSM. RESULTS Furanodiene, curdione and germacrone had a good linearity rangings respectively(R² ≥ 0.999 9). RCFs and RRTs of furanodiene and curdione were stable. There was no significant difference between the results of SRSM and ESM. CONCLUSION The developed SRSM is simple and stable, which can determine the contents above mentioned in the Zedoary Turmeric oil instead of the ESM.