前胡香豆素类成分HPLC指纹图谱研究

HPLC Fingerprint of Coumarins of Peucedani Radix

关键词:  前胡  香豆素类成分  指纹图谱

Key words:  Peucedani Radix  coumarins  fingerprint

摘要:

目的 建立前胡香豆素类成分的HPLC指纹图谱，为科学评价其质量提供可靠方法。方法 采用Ecosil-C₁₈色谱柱（4.6 mm×250 mm，5 μm），以甲醇-水（68：32）为流动相，分析时间50 min，流速1.0 mL·min^-1，柱温40℃，检测波长323 nm；采用LC-MS对前胡香豆素类成分进行解析，并对特征峰进行归属；运用中药色谱指纹图谱相似度评价系统软件，对18批前胡样品和4批混淆品进行相似度评价。结果 以白花前胡甲素和白花前胡乙素为参照物，建立了以8个共有峰为指标成分的前胡药材指纹图谱，通过LC-MS定性分析结合对照品比对，推测8个共有峰为前胡香豆素D、前胡香豆素E、peucedanumarin Ⅱ、白花前胡甲素、peucedanumarin Ⅰ、白花前胡乙素、白花前胡素E及其同分异构体，并且以相似度为0.9能很好地区分前胡正品和混淆品。结论 该方法简单、重复性良好，可为前胡药材的质量评价提供有效鉴别方法。

Abstract:

OBJECTIVE To establish the method of HPLC fingerprint analysis of coumarins for the quality control of Peucedani Radix. METHODS The HPLC fingerprint were obtained on an Ecosil-C₁₈(4.6 mm×250 mm, 5 μm) with the isocratic elution solvent system composed of methanol and water(68:32). The analysis time was 50 min, the flow rate was 1.0 mL·min^-1, the column temperature was maintained at 40℃, and the detection wavelength was set at 323 nm. LC-MS was used to analyze the composition of adulterants of Peucedani Radix, and to identified the characteristic peaks. Similarity of 18 batches of Peucedani Radix samples and 4 batches of adulterants were evaluated by chromatographic fingerprint similarity evaluation software. RESULTS The HPLC fingerprint of Peucedani Radix was established based on the reference of praeruptorin A and praeruptorin B. The detected peaks preliminarily belonged to qianhucoumarin D, qianhucoumarin E, peucedanumarin II, praeruptorin A, peucedanumarin I, praeruptorin B, praeruptorin E and its isomer by qualitative analysis of LC-MS combined with reference comparison. In addition, the similarity was 0.9, which could distinguish Peucedani Radix and its confusion well. CONCLUSION The method is simple and reproducible, and can provide an effective identification method for the quality evaluation of Peucedani Radix.