牛蒡苷元对脑缺血再灌注大鼠的保护作用

Protective Effects of Arctigenin on Rats with Cerebral Ischemia Reperfusion

关键词:  牛蒡苷元  脑缺血再灌注  氧化应激  炎症反应

Key words:  arctigenin  cerebral ischemia reperfusion  oxidative stress  inflammatory reaction

摘要:

目的 研究牛蒡苷元对脑缺血再灌注大鼠的保护作用机制。方法 将90只SD大鼠随机分为6组，空白对照组、假手术组、模型组和25，50，100 mg·kg^-1牛蒡苷元组，每组15只，根据组别分别进行相应的给药干预。采用改良线栓法建立大鼠脑缺血再灌注模型，造模结束后，对大鼠的生存情况及神经功能缺损评分进行评价；脑缺血再灌注24 h后，随机每组抽取2只大鼠对其大脑组织进行病理检测，其余大鼠处死后沿缺血灶剥离缺血半暗带脑组织，ELISA法检测各组缺血半暗带脑组织中的超氧化物歧化酶（SOD）、丙二醛（MDA）、乳酸脱氢酶（LDH）、白介素1β（IL-1β）、白介素6（IL-6）、肿瘤坏死因子α（TNF-α）的含量。结果 与模型组相比，给予50，100 mg·kg^-1牛蒡苷元干预后，大鼠生存情况较好，神经功能缺损评分明显降低（P<0.01），病理切片显示2组梗死灶周围炎性细胞数量显著减少，缺血半暗带脑组织中SOD含量显著升高（P<0.01），MDA、LDH、IL-1β、IL-6以及TNF-α含量明显降低（P<0.01）；给予25 mg·kg^-1牛蒡苷元后，大鼠梗死灶周围炎性细胞数量、LDH、IL-1β、IL-6以及TNF-α含量与模型组比较差异无统计学意义，SOD含量明显升高（P<0.05），MDA含量明显降低（P<0.05）。结论 牛蒡苷元能从抗氧化与抗炎方面发挥对脑缺血再灌注大鼠的保护作用。

Abstract:

OBJECTIVE To study the protective effect of arctigenin on rats with cerebral ischemia reperfusion. METHODS Ninety SD rats were randomly divided into 6 groups:blank control group, sham operation group, model group, and arctigenin groups(25, 50 and 100 mg·kg^-1); 15 rats in each group, and different medication intervention were given according to the groups. An improved line-bolt method was used to establish a rat cerebral ischemia-reperfusion model, after the rats' model established successfully, the survival condition and nerve function defect score of the rats were evaluated. After 24 h of cerebral ischemia-reperfusion, two rats were randomly selected and sacrificed for pathological examination of their brain tissue, the ischemic penumbra of the rest rats was stripped off and the levels of SOD, MDA, LDH, IL-1β, IL-6 and TNF-α were detected. RESULTS Compared with the model group, the survival condition of the rats was better and the scores of nerve function defect were significantly lower after intervened with 50, 100 mg·kg^-1 of arctigenin(P<0.01), the number of inflammatory cells around the infarct were significantly decreased, the level of SOD in ischemic penumbra were significantly increased(P<0.01), while the levels of MDA, LDH, IL-1β, IL-6 and TNF-α were significantly decreased. After administration of 25 mg·kg^-1 arctigenin, the number of inflammatory cells around the infarct and the levels of LDH, IL-1β, IL-6, TNF-α were not statistically different from those in the model group, the level of SOD increased significantly(P<0.05), and the levels of MDA was decreased significantly(P<0.05). CONCLUSION Arctigenin can protect cerebral ischemia reperfusion rats through anti-oxidation and anti-inflammatory effects.