人参与五灵脂配伍对人参皂苷含量的影响

Effects of Compatibility Between Panax Ginseng and Trogopterus Dung on the Ginsenosides Content

关键词:  人参  红参  生晒参  五灵脂  人参皂苷  高效液相色谱法

Key words:  panax ginseng  red ginseng  white ginseng  trogopterus dung  ginsenosides  HPLC

摘要:

目的 通过测定人参、红参、生晒参与五灵脂以不同比例配伍后人参皂苷的含量，探讨"人参畏五灵脂"的作用机制。方法 采用Agilent Athena C₁₈色谱柱（4.6 mm×250 mm，5 μm），以乙腈（A）-磷酸盐缓冲液（B，pH 6.8）为流动相，进行梯度洗脱，流速1.0 mL·min^-1，柱温25℃，检测波长203 nm。结果 人参皂苷Rg₁、Re、Rf、Rb₁、Rc、Rb₂、Rd进样量分别在0.03 6 2~1.16 μg（r=0.998 5）、0.037 1~1.19 μg（r=0.999 0）、0.030 3~0.97 μg（r=0.991 4）、0.035 0~1.12 μg（r=0.993 9）、0.030 6~0.98 μg（r=0.997 3）、0.030 3~0.97 μg（r=0.999 1）、0.031 2~1.00 μg（r=0.999 1）内呈良好的线性关系；加样回收率RSD均<3%，方法的稳定性、重复性和精密度均良好（RSD<3%）。结论 人参、红参、生晒参与五灵脂配伍后7种人参皂苷含量显著下降，不同炮制方法的人参五灵脂配伍后，在比例为1：4时，人参皂苷Rb₁、Rb₂下降最大，达到90%，人参皂苷Rg₁含量减少最小，但也下降了70%以上。

Abstract:

OBJECTIVE To investigate the action mechanism by detecting the ginsenoside content changes in panax genseng, red ginseng, white ginseng with different proportions of trogopterus dung. METHODS The separation was performed on Agilent Athena C₁₈(4.6 mm×250 mm, 5 μm) column and the mobile phase consisted of acetonitrile(A) and H₃PO₄(B, pH6.8) water solution in gradient elution at flow rate of 1.0 mL·min^-1, the column temperature was 25℃, the detection wavelength was set at 203 nm. RESULTS The linear ranges were 0.036 2-1.16 μg(r=0.998 5) for Rg₁, 0.037 1-1.19 μg(r=0.999 0) for Re, 0.030 3-0.97 μg(r=0.991 4) for Rf, 0.035 0-1.12 μg(r=0.993 9) for Rb₁, 0.030 6-0.98 μg(r=0.997 3) for Rc, 0.030 3-0.97 μg (r=0.999 1) for Rb₂, 0.031 2-1.00 μg(r=0.999 1) for Rd. The sample recovery rate RSD <3%, the stability, repeatability and precision of the method were good(RSD <3%). CONCLUSION The contents of seven ginsenoside decreased significantly when panax ginseng, red ginseng, white ginseng combined with trogopterus dung, and when the ratio was 1:4, Rb₁ and Rb₂ decreased the maximum, highest fall achieve 90%. The content of Rg₁ reduced the minimum, but also fell more than 70%.